Author Choi JB, Uchino H, Azuma K, Iwashita N, Tanaka Y, Mochizuki H, Migita M, Shimada T, Kawamori R, Watada H.
Title Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta cells.
Journal Diabetologia
Abstract AIMS/HYPOTHESIS:Bone marrow cells contain at least two distinct types of stem cells which are haematopoietic stem cells and mesenchymal stem cells. Both cells have the ability to differentiate into a variety of cell types derived from all three germ layers. Thus, bone marrow stem cells could possibly be used to generate new pancreatic beta cells for the treatment of diabetes. In this study, we investigated the feasibility of bone marrow-derived cells to differentiate into beta cells in pancreas.
METHODS:Using green fluorescent protein transgenic mice as donors, the distribution of haematogenous cells in the pancreas was studied after bone marrow transplantation.
RESULTS:In the pancreas of green fluorescent protein chimeric mice, green fluorescent protein-positive cells were found in the islets, but none of these cells expressed insulin. Previous data has suggested that tissue injury can recruit haematopoietic stem cells or their progeny to a non-haematopietic cell fate. Therefore, low-dose streptozotocin (30 or 50 mg/kg on five consecutive days) was injected into the mice 5 weeks after bone marrow transplantation, but no green fluorescent protein-positive cells expressing insulin were seen in the islets or around the ducts of the pancreas.
CONCLUSIONS/INTERPRETATION:Our data suggests that bone marrow-derived cells are a distinct cell population from islet cells and that transdifferentiation from bone marrow-derived cells to pancreatic beta cells is rarely observed.
Volume 46(10)
Pages 1366-74
Published 2003-10
DOI 10.1007/s00125-003-1182-9
PMID 12898006
MeSH Animals Bone Marrow Cells / cytology* Bone Marrow Cells / metabolism Bone Marrow Transplantation* Cell Differentiation Chimera Feasibility Studies Green Fluorescent Proteins Indicators and Reagents Insulin / metabolism Islets of Langerhans / cytology* Islets of Langerhans / drug effects Islets of Langerhans / metabolism Luminescent Proteins / genetics Mice Mice, Transgenic / genetics Pancreas / physiology* Streptozocin / pharmacology
IF 7.113
Times Cited 146
Mice C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb