RRC ID 1005
Author Abliz P, Fukushima K, Takizawa K, Miyaji M, Nishimura K.
Title Specific oligonucleotide primers for identification of Hortaea werneckii, a causative agent of tinea nigra.
Journal Diagn Microbiol Infect Dis
Abstract Hortaea werneckii, a black yeast-like hyphomycete that is widely distributed in tropical and subtropical environments, can cause superficial mycotic infection in humans. This fungus was recently isolated from superficial infectious lesions of a guinea pig in Japan. An oligonucleotide primer set specific for Hortaea werneckii was designed on the basis of the internal transcribed spacer regions of the ribosomal DNA (rDNA). Polymerase chain reaction (PCR) with this primer set yielded a 306 bp PCR amplicon from only H. werneckii. This primer set did not amplify DNAs of 42 other related dematiaceous species, including the medically important dematiaceous fungi Cladophialophora carrionii, Fonsecaea pedrosoi, Phialophora verrucosa, and Exophiala dermatitidis, and eight species of medically important yeasts, including Candida (C.) albicans, C. dublinensis, C. glabrata, C. parapsilosis, C. tropicalis, Cryptococcus neoformans var. neoformans, Malassezia furfur, and Trichosporon asahii var. asahii. PCR with this primer set may be a useful technique for rapid identification of H. werneckii.
Volume 46(2)
Pages 89-93
Published 2003-6-1
DOI 10.1016/s0732-8893(03)00035-x
PII S073288930300035X
PMID 12812723
MeSH Base Sequence DNA Primers* DNA, Ribosomal / analysis Dermatomycoses / diagnosis* Exophiala / classification* Humans Molecular Sequence Data Mycological Typing Techniques Polymerase Chain Reaction / methods* Sampling Studies Sensitivity and Specificity Tinea / diagnosis*
IF 2.499
Times Cited 15
WOS Category INFECTIOUS DISEASES MICROBIOLOGY
Resource
Pathogenic microorganisms IFM 4802 IFM 4805 IFM 4818 IFM 4883 IFM 4886 IFM 4887 IFM 4889 IFM 4890 IFM 4922 IFM 4923 IFM 4924 IFM 4925 IFM 4926 IFM 4928 IFM 4930 IFM 4931 IFM 4936 IFM 4939 IFM 4987 IFM 4988 IFM 4989 IFM 4990 IFM 40214 IFM 40216 IFM 40703 IFM 41348 IFM 41446 IFM 41447 IFM 41451 IFM 41452 IFM 41453 IFM 41454 IFM 41456 IFM 41458 IFM 41479 IFM 41500 IFM 41537 IFM 41540 IFM 41542 IFM 41577 IFM 41578 IFM 41641 IFM 41654 IFM 41691 IFM 41710 IFM 41818 IFM 41828 IFM 41871 IFM 45106 IFM 45989 IFM 46165 IFM 46816 IFM 46863 IFM 46888 IFM 48313 IFM 48429 IFM 48585 IFM 51361 IFM 51362 IFM 51363 IFM 51364 IFM 51365 IFM 51366 IFM 51367 IFM 51368 IFM 51369 IFM 51370 IFM 51371 IFM 51372 IFM 51373 IFM 51425