RRC ID 1007
著者 Yamaguchi M, Biswas SK, Suzuki Y, Furukawa H, Takeo K.
タイトル Three-dimensional reconstruction of a pathogenic yeast Exophiala dermatitidis cell by freeze-substitution and serial sectioning electron microscopy.
ジャーナル FEMS Microbiol Lett
Abstract The structure of a budding cell of the pathogenic yeast Exophiala dermatitidis was observed in three dimensions after freeze-substitution, serial ultrathin sectioning and computer reconstruction. The nucleus occupied about 10% of the cell volume. The spindle pole body was composed of two disk elements connected by an intervening midpiece, and occupied about 0.01% of the cell volume. The cell wall consisted of an inner transparent layer, a middle electron-opaque layer, and an outer fibrous layer. The mitochondria occupied about 10% of the cell volume. There were numerous mitochondria in the mother cell and the bud, but no 'giant mitochondrion' was seen. The ratio of mitochondrial volume within the bud to the mitochondrial volume of the cell was close to the ratio of bud:cell cytoplasmic volume. The results emphasize the importance of good cryofixation for 'perfect' preservation of yeast cell structure.
巻・号 219(1)
ページ 17-21
公開日 2003-2-14
DOI 10.1016/S0378-1097(02)01181-3
PII S0378109702011813
PMID 12594017
MeSH Exophiala / pathogenicity Exophiala / ultrastructure* Freeze Substitution / methods* Humans Image Processing, Computer-Assisted / methods* Microscopy, Electron / methods Microtomy / methods*
IF 1.987
引用数 20
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 IFM 4844