RRC ID 1023
Author Huaman MC, Sato Y, Aguilar JL, Terashima A, Guerra H, Gotuzzo E, Kanbara H.
Title Gelatin particle indirect agglutination and enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis using Strongyloides venezuelensis antigen.
Journal Trans R Soc Trop Med Hyg
Abstract Routine microscopical examination of stool specimens for diagnosis of strongyloidiasis is insensitive and serological methods using Strongyloides stercoralis antigen are at present not available for field studies. We evaluated 2 techniques, enzyme-linked immunosorbent assay (ELISA) and gelatin particle indirect agglutination (GPIA), using an antigen obtained from the rodent parasite, S. venezuelensis. Fifty-four Peruvian patients with different clinical forms of strongyloidiasis were studied: 12 asymptomatic, 31 symptomatic, and 11 hyperinfection cases. Our results demonstrate that both ELISA and GPIA using S. venezuelensis antigen are useful for diagnosis of strongyloidiasis, with sensitivities of 74.1% and 98.2%, respectively and a specificity of 100% for both techniques. We found that GPIA is a highly sensitive test for patients with suspected chronic infection and/or hyperinfection. In the hyperinfection cases, significantly lower concentrations of specific immunoglobulin antibodies and eosinophils (P < 0.001) were found compared with the asymptomatic and symptomatic cases.
Volume 97(5)
Pages 535-8
Published 2003-1-1
DOI 10.1016/s0035-9203(03)80017-2
PMID 15307419
MeSH Adolescent Adult Aged Agglutination Tests / methods* Agglutination Tests / standards Analysis of Variance Animals Antigens, Helminth / analysis Child Child, Preschool Enzyme-Linked Immunosorbent Assay / methods* Enzyme-Linked Immunosorbent Assay / standards HTLV-I Infections / complications Humans Middle Aged Sensitivity and Specificity Strongyloides / immunology Strongyloides / isolation & purification Strongyloidiasis / diagnosis*
IF 1.868
Times Cited 16
Pathogenic microorganisms ?