| RRC ID |
1023
|
| 著者 |
Huaman MC, Sato Y, Aguilar JL, Terashima A, Guerra H, Gotuzzo E, Kanbara H.
|
| タイトル |
Gelatin particle indirect agglutination and enzyme-linked immunosorbent assay for diagnosis of strongyloidiasis using Strongyloides venezuelensis antigen.
|
| ジャーナル |
Trans R Soc Trop Med Hyg
|
| Abstract |
Routine microscopical examination of stool specimens for diagnosis of strongyloidiasis is insensitive and serological methods using Strongyloides stercoralis antigen are at present not available for field studies. We evaluated 2 techniques, enzyme-linked immunosorbent assay (ELISA) and gelatin particle indirect agglutination (GPIA), using an antigen obtained from the rodent parasite, S. venezuelensis. Fifty-four Peruvian patients with different clinical forms of strongyloidiasis were studied: 12 asymptomatic, 31 symptomatic, and 11 hyperinfection cases. Our results demonstrate that both ELISA and GPIA using S. venezuelensis antigen are useful for diagnosis of strongyloidiasis, with sensitivities of 74.1% and 98.2%, respectively and a specificity of 100% for both techniques. We found that GPIA is a highly sensitive test for patients with suspected chronic infection and/or hyperinfection. In the hyperinfection cases, significantly lower concentrations of specific immunoglobulin antibodies and eosinophils (P < 0.001) were found compared with the asymptomatic and symptomatic cases.
|
| 巻・号 |
97(5)
|
| ページ |
535-8
|
| 公開日 |
2003-1-1
|
| DOI |
10.1016/s0035-9203(03)80017-2
|
| PMID |
15307419
|
| MeSH |
Adolescent
Adult
Aged
Agglutination Tests / methods*
Agglutination Tests / standards
Analysis of Variance
Animals
Antigens, Helminth / analysis
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay / methods*
Enzyme-Linked Immunosorbent Assay / standards
HTLV-I Infections / complications
Humans
Middle Aged
Sensitivity and Specificity
Strongyloides / immunology
Strongyloides / isolation & purification
Strongyloidiasis / diagnosis*
|
| IF |
1.868
|
| 引用数 |
16
|
|
WOS 分野
|
TROPICAL MEDICINE
PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
|
| リソース情報 |
| 病原微生物 |
|
