RRC ID 1024
Author Biswas D, Itoh K, Sasakawa C.
Title Role of microfilaments and microtubules in the invasion of INT-407 cells by Campylobacter jejuni.
Journal Microbiol. Immunol.
Abstract The internalization mechanisms triggered by Campylobacter jejuni were studied by invasion assays conducted with different inhibitors that act on the cytoskeleton structure of eukaryotic cells. The depolymerization of microfilaments by cytochalasin-D and that of microtubules by colchicines and nocodazole inhibited the uptake of C. jejuni into INT-407 cells in a dose-dependent manner. The inhibitory effect of microfilament depolymerization on C. jejuni internalization was more pronounced than that of microtubule depolymerization. By immunofluorescence microscopic observations, it was demonstrated that both microfilaments and microtubules were localized in INT-407 cells after C. jejuni infection. These data suggest that the internalization mechanism triggered by C. jejuni is associated with the combined effect of microfilaments and microtubules of host cells.
Volume 47(6)
Pages 469-73
Published 2003
PMID 12906108
MeSH Actin Cytoskeleton / physiology* Actins / metabolism Biopolymers Campylobacter jejuni / pathogenicity Campylobacter jejuni / physiology* Cells, Cultured / drug effects Cells, Cultured / ultrastructure Colchicine / pharmacology Cytochalasin D / pharmacology Cytoskeleton / ultrastructure Dose-Response Relationship, Drug Endocytosis / drug effects Endocytosis / physiology* Epithelial Cells / drug effects Epithelial Cells / microbiology Epithelial Cells / ultrastructure Humans Intestines / cytology Intestines / embryology Intestines / microbiology* Intestines / ultrastructure Microscopy, Fluorescence Microtubules / drug effects Microtubules / physiology* Nocodazole / pharmacology Species Specificity Tubulin / metabolism Virulence / physiology
IF 1.335
Times Cited 30
Pathogenic microorganisms C. jejuni?