| RRC ID |
1031
|
| 著者 |
Liu H, Li Y, Huang X, Kawamura Y, Ezaki T.
|
| タイトル |
Use of the dnaJ gene for the detection and identification of all Legionella pneumophila serogroups and description of the primers used to detect 16S rDNA gene sequences of major members of the genus Legionella.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
We sequenced about 930 bp of the dnaJ gene from 15 Legionella pneumophila serogroups and some other members of the genus Legionella. As L. pneumophila 16S rDNA sequences could not discriminate between all subspecies and serogroups, we assessed the use of dnaJ gene sequences to differentiate between Legionella subspecies as well as between L. pneumophila serogroups. A phylogenetic analysis revealed that dnaJ gene sequences were more variable between the L. pneumophila serogroups than mip gene and 16S rDNA sequences. By studying 61 strains from 41 species of the genus Legionella, as well as other genera, we established a PCR method that could amplify 285 bp of dnaJ gene from all L. pneumophila serogroups. This primer set was more sensitive than mip gene primers and was able to detect 0.25 ng of purified DNA. We also describe the 16S rDNA primers that were used to detect most Legionella genus members.
|
| 巻・号 |
47(11)
|
| ページ |
859-69
|
| 公開日 |
2003-1-1
|
| DOI |
10.1111/j.1348-0421.2003.tb03452.x
|
| PMID |
14638997
|
| MeSH |
Bacterial Proteins
DNA Primers
Genetic Variation
HSP40 Heat-Shock Proteins
Heat-Shock Proteins / genetics*
Immunophilins
Legionella pneumophila / classification*
Legionella pneumophila / genetics
Legionella pneumophila / isolation & purification
Membrane Proteins
Peptidylprolyl Isomerase*
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S / analysis*
RNA, Ribosomal, 16S / chemistry
RNA, Ribosomal, 16S / genetics
|
| IF |
1.566
|
| 引用数 |
32
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
GTC 00745
GTC 00746
GTC 00747
GTC 00302(GIFU 9246)?
GTC 00297
GTC 00748
GTC 00750
GTC 00751
GTC 00754
GTC 00757
GTC 00756
GTC 00804
GTC 00805
GTC 00806
GTC 00807
GTC 00697?
GTC 00464?
GTC 00515
GTC 00374
GTC 00477?
GTC 00749(GIFU 9244)?
GTC 00698?
GTC 00322?
GTC 00703
GTC 00300?
GTC 00699?
GTC 00372(GIFU 10740)?
GTC 00411(GIFU 11367)?
GTC 00373(GIFU 10741)?
GTC 00741?
GTC 00301(GIFU 9245)?
GTC 00299?
GTC 00513
GTC 00742
GTC 00319(GIFU 10061)?
GTC 00444?
GTC 00302
GTC 00296
GTC 00702
GTC 00743?
GTC 00512?
GTC 11746(GIFU 11746)?
GTC 00701?
GTC 11747(GIFU 11747)?
GTC 00516?
GTC 01737
GTC 00744
GTC 11962( GIFU 11962)?
GTC 00013
GTC 00008. GTC 00503
GTC 00002
GTC 00120 |
