RRC ID 1034
著者 Sago T, Mori Y, Takagi H, Iwata H, Murase K, Kawamura Y, Hirose H.
タイトル Local treatment of Dacron patch graft contaminated with Staphylococcus aureus with antibiotic-releasing porous apatite ceramic: an experimental study in the rabbit.
ジャーナル J Vasc Surg
Abstract OBJECTIVE:The purpose of this study was to investigate whether or not infection of prosthetic vascular graft could be prevented with the placement of antibiotic-loaded porous apatite ceramic on the graft with bacterial contamination.
METHODS:Teicoplanin as an antibiotic was loaded into a beta-tricalcium phosphate (TCP) block of porous apatite ceramic. The activity of teicoplanin released from teicoplanin-loaded TCP was examined for 40 days at 2-day intervals. In an in vivo study, the graft was patched in the abdominal aorta of a rabbit. Two different doses of Staphylococcus aureus were applied (n = 13). Teicoplanin-loaded TCP was placed around the graft infected with S aureus (n = 6). The graft, the tissue around it, and the arterial blood were collected and cultivated 4 weeks after operation.
RESULTS:The activity of teicoplanin in the replaced saline solution was maintained for at least 40 days. Because graft infection was established in all rabbits with a dose of 2 x 10(7) colony forming units, this dose was used as the dose of S aureus in this study. Fifty-seven percent of the subjects died from the infection. When teicoplanin-loaded TCP was placed around the graft contaminated with S aureus, no rabbits died. Bacterial cultures of the graft and of tissues around it were negative in all rabbits killed 4 weeks after operation. This infection was prevented with teicoplanin-loaded TCP in all rabbits.
CONCLUSION:Teicoplanin-loaded TCP may be useful in avoiding Dacron graft infection with S aureus in rabbits.
巻・号 37(1)
ページ 169-74
公開日 2003-1-1
DOI 10.1067/mva.2003.105
PII S0741521402752464
PMID 12514596
MeSH Animals Anti-Bacterial Agents / administration & dosage* Apatites* Blood Vessel Prosthesis* Ceramics* Equipment Contamination / prevention & control* Male Patch Tests Polyethylene Terephthalates* Prosthesis-Related Infections / prevention & control Rabbits Staphylococcal Infections / prevention & control Staphylococcus aureus* Teicoplanin / administration & dosage
IF 3.405
引用数 13
WOS 分野 SURGERY PERIPHERAL VASCULAR DISEASE
リソース情報
病原微生物 GIFU 12173?