論文 - 詳細
RRC ID | 1034 |
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著者 | Sago T, Mori Y, Takagi H, Iwata H, Murase K, Kawamura Y, Hirose H. |
タイトル | Local treatment of Dacron patch graft contaminated with Staphylococcus aureus with antibiotic-releasing porous apatite ceramic: an experimental study in the rabbit. |
ジャーナル | J Vasc Surg |
Abstract |
OBJECTIVE:The purpose of this study was to investigate whether or not infection of prosthetic vascular graft could be prevented with the placement of antibiotic-loaded porous apatite ceramic on the graft with bacterial contamination. METHODS:Teicoplanin as an antibiotic was loaded into a beta-tricalcium phosphate (TCP) block of porous apatite ceramic. The activity of teicoplanin released from teicoplanin-loaded TCP was examined for 40 days at 2-day intervals. In an in vivo study, the graft was patched in the abdominal aorta of a rabbit. Two different doses of Staphylococcus aureus were applied (n = 13). Teicoplanin-loaded TCP was placed around the graft infected with S aureus (n = 6). The graft, the tissue around it, and the arterial blood were collected and cultivated 4 weeks after operation. RESULTS:The activity of teicoplanin in the replaced saline solution was maintained for at least 40 days. Because graft infection was established in all rabbits with a dose of 2 x 10(7) colony forming units, this dose was used as the dose of S aureus in this study. Fifty-seven percent of the subjects died from the infection. When teicoplanin-loaded TCP was placed around the graft contaminated with S aureus, no rabbits died. Bacterial cultures of the graft and of tissues around it were negative in all rabbits killed 4 weeks after operation. This infection was prevented with teicoplanin-loaded TCP in all rabbits. CONCLUSION:Teicoplanin-loaded TCP may be useful in avoiding Dacron graft infection with S aureus in rabbits. |
巻・号 | 37(1) |
ページ | 169-74 |
公開日 | 2003-1-1 |
DOI | 10.1067/mva.2003.105 |
PII | S0741521402752464 |
PMID | 12514596 |
MeSH | Animals Anti-Bacterial Agents / administration & dosage* Apatites* Blood Vessel Prosthesis* Ceramics* Equipment Contamination / prevention & control* Male Patch Tests Polyethylene Terephthalates* Prosthesis-Related Infections / prevention & control Rabbits Staphylococcal Infections / prevention & control Staphylococcus aureus* Teicoplanin / administration & dosage |
IF | 3.405 |
引用数 | 13 |
WOS 分野 | SURGERY PERIPHERAL VASCULAR DISEASE |
リソース情報 | |
病原微生物 | GIFU 12173? |