RRC ID 1057
Author Kageyama A, Yazawa K, Mukai A, Kinoshita M, Takata N, Nishimura K, Kroppenstedt RM, Mikami Y.
Title Nocardia shimofusensis sp. nov., isolated from soil, and Nocardia higoensis sp. nov., isolated from a patient with lung nocardiosis in Japan.
Journal Int. J. Syst. Evol. Microbiol.
Abstract Three actinomycete strains isolated from soils and one strain isolated from a patient with lung nocardiosis in 1999 and 2001 in Japan have been provisionally assigned to the genus Nocardia on the basis of morphological criteria. These isolates were further investigated to determine their specific taxonomic status. Detailed chemotaxonomic characterization and 16S rRNA gene sequence analysis of these isolates confirmed that they belong to the genus Nocardia. The 16S rRNA gene sequences of the four strains were most similar to that of Nocardia farcinica. However, the sequence similarity values between these four strains and N. farcinica were <98.9 %. These four strains were susceptible to 5-fluorouracil, and they have the ability to decompose urea, which is a very characteristic trait. Furthermore, DNA-DNA relatedness data revealed that IFM 10311(T), IFM 10312 and IFM 10313 comprise a single novel species of Nocardia, that IFM 10084(T) represents another novel species of Nocardia and that these two novel species could be distinguished from N. farcinica. The names Nocardia shimofusensis sp. nov. and Nocardia higoensis sp. nov. are proposed, with IFM 10311(T) (=NBRC 100134(T)=JCM 12122(T)=DSM 44733(T)) and IFM 10084(T) (=NBRC 100133(T)=JCM 12121(T)=DSM 44732(T)) as the respective type strains.
Volume 54(Pt 6)
Pages 1927-31
Published 2004-11
DOI 10.1099/ijs.0.63061-0
PII 54/6/1927
PMID 15545412
MeSH Aged Antimetabolites / pharmacology Bacterial Typing Techniques DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Fatty Acids / analysis Fatty Acids / isolation & purification Female Fluorouracil / pharmacology Genes, rRNA Humans Japan Lung / microbiology* Molecular Sequence Data Nocardia / chemistry Nocardia / classification* Nocardia / drug effects Nocardia / genetics Nocardia / isolation & purification* Nocardia / metabolism Nocardia Infections / microbiology* Nucleic Acid Hybridization Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology*
IF 1.932
Times Cited 8
Pathogenic microorganisms IFM 0138 IFM 0228 IFM 0284 IFM 10084(JCM 12121) IFM 10311(JCM 12122) IFM 10312 IFM 10313 JCM 10666?