| RRC ID |
106
|
| 著者 |
Ohyama M, Hirayama Y, Tanuma J, Hirano M, Semba I, Shisa H, Hiai H, Sugihara K, Kitano M.
|
| タイトル |
Expressions of junB and c-fos are enhanced in 4-nitroquinoline 1-oxide-induced rat tongue cancers.
|
| ジャーナル |
Pathol Int
|
| Abstract |
Activator protein-1 (AP-1) is a transcription factor activated in many tumors. Using 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue cancers (TC), the present study investigated the expression levels of genes that encode the components of AP-1, the jun gene family (c-jun, junB and junD) and the fos gene family (c-fos, fra-1, fra-2 and fosB). Expression levels of junB and c-fos mRNAs in TC were significantly elevated compared with those in epithelial tissue of control rat tongue, although only c-fos mRNA levels tended to be elevated in dysplastic tongue epithelium. Histologically, all 4NQO-induced rat TC were well-differentiated squamous cell carcinomas. Immunostaining for JunB and c-Fos proteins was positive in the nuclei of tumor cells of all TC. It is noteworthy that JunB was negative, but c-Fos was positive in the dysplastic tongue epithelium of the 4NQO-treated rats. Immunostaining for both proteins was negative in tongue mucosal epithelium of control rats. There were no mutations in the coding regions of either junB or c-fos in all the TC examined. These results suggest the possibility that the expressions of junB and c-fos were enhanced stepwise in 4NQO-induced carcinogenesis of rat tongue, and that the coexpression of JunB and c-Fos might play an important role in the establishment of TC.
|
| 巻・号 |
54(1)
|
| ページ |
35-40
|
| 公開日 |
2004-1-1
|
| DOI |
10.1046/j.1440-1827.2003.01587.x
|
| PII |
1587
|
| PMID |
14674993
|
| MeSH |
4-Nitroquinoline-1-oxide / toxicity*
Animals
Biomarkers, Tumor / analysis
Carcinoma, Squamous Cell / chemically induced
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / pathology
Cell Nucleus / chemistry
Cell Nucleus / pathology
Disease Models, Animal
Gene Expression Regulation, Neoplastic*
Genes, fos / genetics*
Proto-Oncogene Proteins c-jun / genetics*
RNA, Messenger / metabolism
RNA, Neoplasm / analysis
Rats
Rats, Inbred Strains
Tongue / chemistry
Tongue / drug effects
Tongue / pathology
Tongue Neoplasms / chemically induced
Tongue Neoplasms / genetics*
Tongue Neoplasms / pathology
|
| IF |
2.11
|
| 引用数 |
10
|
|
WOS 分野
|
PATHOLOGY
|
| リソース情報 |
| ラット |
|
