| RRC ID |
10753
|
| 著者 |
Miki Y, Akimoto J, Hiranuma M, Fujiwara Y.
|
| タイトル |
Effect of talaporfin sodium-mediated photodynamic therapy on cell death modalities in human glioblastoma T98G cells.
|
| ジャーナル |
J Toxicol Sci
|
| Abstract |
While photodynamic therapy (PDT) is an effective treatment for glioma, induction of apoptotic cell death of glioma cells is important for ensuring efficacy and safety of PDT treatment in glioma patients, as necrotic cell death can induce late appearance of obstacles in treatment. Here, we investigated the relationship between type of cell death and PDT treatment conditions involved in laser and photosensitizer dosage in human glioblastoma T98G cells. Photosensitizer talaporfin sodium-mediated PDT (NPe6-PDT) treatment induced laser and NPe6 dose-dependent cell death in T98G cells, whereas almost all cells pretreated with NPe6 at ≥ 30 µg/mL were killed by laser irradiation, regardless of laser dose. Morphological analysis showed that combination of high doses of NPe6 and laser irradiation changes the dominant cell death process from apoptosis to necrosis. Biochemical analysis (detection of caspase-3 activity and staining of cell surface-exposed phosphatidylserine) also showed that increasing laser dose changes the type of cell death from apoptotic to necrotic cell death after high-dose treatment with NPe6. Lactate dehydrogenase leakage assay demonstrated that a laser dose of 5 J/cm(2) induced less leakage than 30 J/cm(2). Our results suggested that type of glioma cell death in NPe6-PDT changed with fluctuations in laser and NPe6 dose, and that combination of 30 µg/mL NPe6 with 5 J/cm(2) laser is the best treatment condition for inducing an increase in apoptotic cells while keeping rate of necrotic cell death low in this in vitro study.
|
| 巻・号 |
39(6)
|
| ページ |
821-7
|
| 公開日 |
2014-1-1
|
| DOI |
10.2131/jts.39.821
|
| PII |
DN/JST.JSTAGE/jts/39.821
|
| PMID |
25374373
|
| MeSH |
Antineoplastic Agents / therapeutic use
Antineoplastic Agents / toxicity*
Cell Death / drug effects
Cell Death / radiation effects
Glioblastoma / drug therapy
Glioblastoma / pathology*
Humans
Lasers*
Photochemotherapy*
Photosensitizing Agents / pharmacology
Photosensitizing Agents / therapeutic use
Photosensitizing Agents / toxicity*
Porphyrins / therapeutic use
Porphyrins / toxicity*
Radiation Dosage
Tumor Cells, Cultured
|
| 引用数 |
21
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
T98G |
