RRC ID 10754
著者 Okano K, Yu D, Matsui A, Maezawa Y, Hosokawa Y, Kira A, Matsubara M, Liau I, Tsubokawa H, Masuhara H.
タイトル Induction of cell-cell connections by using in situ laser lithography on a perfluoroalkyl-coated cultivation platform.
ジャーナル Chembiochem
Abstract This article describes a novel laser-directed microfabrication method carried out in aqueous solution for the organization of cell networks on a platform. A femtosecond (fs) laser was applied to a platform culturing PC12, HeLa, or normal human astrocyte (NHA) cells to manipulate them and to facilitate mutual connections. By applying an fs-laser-induced impulsive force, cells were detached from their original location on the plate, and translocated onto microfabricated cell-adhesive domains that were surrounded with a cell-repellent perfluoroalkyl (R(f)) polymer. Then the fs-laser pulse-train was applied to the R(f) polymer surface to modify the cell-repellent surface, and to make cell-adhesive channels of several μm in width between each cell-adhesive domain. PC12 cells elongated along the channels and made contact with others cells. HeLa and NHA cells also migrated along the channels and connected to the other cells. Surface analysis by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) confirmed that the R(f) polymer was partially decomposed. The method presented here could contribute not only to the study of developing networks of neuronal, glial, and capillary cells, but also to the quantitative analysis of nerve function.
巻・号 12(5)
ページ 795-801
公開日 2011-3-21
DOI 10.1002/cbic.201000497
PMID 21341350
MeSH Animals Astrocytes / cytology Cell Adhesion Cell Communication* Cell Line Cell Movement Coated Materials, Biocompatible / chemistry Coated Materials, Biocompatible / metabolism* Equipment Failure Fluorocarbons / chemistry Fluorocarbons / metabolism* HeLa Cells Humans Lasers Microtechnology / methods* PC12 Cells Rats Surface Properties Tissue Array Analysis / instrumentation*
IF 2.576
引用数 13
WOS 分野 CHEMISTRY, MEDICINAL BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 PC-12(RCB0009) HeLa(RCB0007)