RRC ID |
1076
|
著者 |
Watanabe A, Kamei K, Sekine T, Waku M, Nishimura K, Miyaji M, Tatsumi K, Kuriyama T.
|
タイトル |
Effect of aeration on gliotoxin production by Aspergillus fumigatus in its culture filtrate.
|
ジャーナル |
Mycopathologia
|
Abstract |
Gliotoxin, one of the mycotoxins produced by Aspergillus fumigatus, has various, potent bioactivities. However, it has not been considered to be a toxic (or virulence) factor because of its slow production. The aim of the present study was to investigate the effects of aeration on the cytotoxicity of A. fumigatus culture filtrate, and to determine the optimal condition for the rapid production of gliotoxin from this fungus. Fungal culture filtrates were made in three different containers under various conditions of aeration and O2 concentration. These filtrates were compared in terms of their cytotoxicity on murine macrophages and analyzed by gas chromatography. The culture filtrate showed high cytotoxicity when it was made under highly aerated conditions, but it was significantly less cytotoxic when prepared under non-aerated conditions. The cytotoxic activity became evident within 15 h of culture at 20% O2, when the fungus had already started producing gliotoxin. The culture filtrates also contained some other as yet unidentified substances that might also to some extent contribute to the cytotoxicity. In light of these results, the authors propose that a highly aerated condition is responsible for the rapid production of gliotoxin, and that gliotoxin might play an important role in the respiratory infection by A. fumigatus, with other toxic substances acting additively or synergistically.
|
巻・号 |
157(3)
|
ページ |
245-54
|
公開日 |
2004-4-1
|
PMID |
15180150
|
MeSH |
Aerobiosis
Animals
Aspergillus fumigatus / growth & development
Aspergillus fumigatus / metabolism*
Colorimetry
Gas Chromatography-Mass Spectrometry
Gliotoxin / biosynthesis*
Macrophages, Peritoneal / microbiology*
Male
Mice
Oxygen / metabolism
Oxygen / pharmacology
Tetrazolium Salts / metabolism
|
IF |
2.452
|
引用数 |
14
|
WOS 分野
|
MYCOLOGY
|
リソース情報 |
病原微生物 |
IFM 41362
IFM 47439
IFM 47450
IFM 49824 |