Reference - Detail
| RRC ID | 10774 |
|---|---|
| Author | Matsuda H, Akaki J, Nakamura S, Okazaki Y, Kojima H, Tamesada M, Yoshikawa M. |
| Title | Apoptosis-inducing effects of sterols from the dried powder of cultured mycelium of Cordyceps sinensis. |
| Journal | Chem Pharm Bull (Tokyo) |
| Abstract |
The methanolic extract and its ethyl acetate-soluble fraction from the dried powder of cultured Cordyceps sinensis mycelium showed cytotoxic effects on promyelocytic leukemia HL-60 cells. Sitosterol (1), 5 alpha,8 alpha-epidioxy-22E-ergosta-6,22-dien-3beta-ol (2), 5 alpha,8 alpha-epidioxy-22E-ergosta-6,9(11),22-trien-3beta-ol (3), 5 alpha,6 alpha-epoxy-5 alpha-ergosta-7,22-dien-3beta-ol (4), and ergosterol (5) were isolated from the ethyl acetate fraction. Among the isolated compounds, 2-4 with a peroxide ring or an epoxide ring showed substantial cytotoxic activity with IC(50) values of 7.3-7.8 microg/ml, but 1 and 5 showed only moderate activity. Furthermore, apoptosis of HL-60 cells was observed 24 h after treatment with 2-4 (10, 20 microg/ml) using the TdT-mediated dUTP nick-end labeling method, and their apoptosis-inducing activities are suggested to be dependent on the activation of caspases-3/7. To the best of our knowledge, this is the first report of the isolation of sterol constituents (1-5) from cultured C. sinensis mycelium. |
| Volume | 57(4) |
| Pages | 411-4 |
| Published | 2009-4-1 |
| DOI | 10.1248/cpb.57.411 |
| PII | JST.JSTAGE/cpb/57.411 |
| PMID | 19336939 |
| MeSH | Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Apoptosis / drug effects* Cordyceps / chemistry* HL-60 Cells Humans Molecular Structure Mycelium / chemistry Powders Sterols / isolation & purification Sterols / pharmacology* |
| IF | 1.416 |
| Times Cited | 22 |
| WOS Category | PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL CHEMISTRY, MULTIDISCIPLINARY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HL60(RCB0041) |