論文 - 詳細
| RRC ID | 10787 |
|---|---|
| 著者 | Murakami M, Kambe T, Shimbara S, Yamamoto S, Kuwata H, Kudo I. |
| タイトル | Functional association of type IIA secretory phospholipase A(2) with the glycosylphosphatidylinositol-anchored heparan sulfate proteoglycan in the cyclooxygenase-2-mediated delayed prostanoid-biosynthetic pathway. |
| ジャーナル | J Biol Chem |
| Abstract |
An emerging body of evidence suggests that type IIA secretory phospholipase A(2) (sPLA(2)-IIA) participates in the amplification of the stimulus-induced cyclooxygenase (COX)-2-dependent delayed prostaglandin (PG)-biosynthetic response in several cell types. However, the biological importance of the ability of sPLA(2)-IIA to bind to heparan sulfate proteoglycan (HSPG) on cell surfaces has remained controversial. Here we show that glypican, a glycosylphosphatidylinositol (GPI)-anchored HSPG, acts as a physical and functional adaptor for sPLA(2)-IIA. sPLA(2)-IIA-dependent PGE(2) generation by interleukin-1-stimulated cells was markedly attenuated by treatment of the cells with heparin, heparinase or GPI-specific phospholipase C, which solubilized the cell surface-associated sPLA(2)-IIA. Overexpression of glypican-1 increased the association of sPLA(2)-IIA with the cell membrane, and glypican-1 was coimmunoprecipitated by the antibody against sPLA(2)-IIA. Glypican-1 overexpression led to marked augmentation of sPLA(2)-IIA-mediated arachidonic acid release, PGE(2) generation, and COX-2 induction in interleukin-1-stimulated cells, particularly when the sPLA(2)-IIA expression level was suboptimal. Immunofluorescent microscopic analyses of cytokine-stimulated cells revealed that sPLA(2)-IIA was present in the caveolae, a microdomain in which GPI-anchored proteins reside, and also appeared in the perinuclear area in proximity to COX-2. We therefore propose that a GPI-anchored HSPG glypican facilitates the trafficking of sPLA(2)-IIA into particular subcellular compartments, and arachidonic acid thus released from the compartments may link efficiently to the downstream COX-2-mediated PG biosynthesis. |
| 巻・号 | 274(42) |
| ページ | 29927-36 |
| 公開日 | 1999-10-15 |
| DOI | 10.1074/jbc.274.42.29927 |
| PII | S0021-9258(19)51848-8 |
| PMID | 10514475 |
| MeSH | Cell Line Cyclooxygenase 2 Dinoprostone / biosynthesis* Glycosylphosphatidylinositols / metabolism* Group II Phospholipases A2 Heparan Sulfate Proteoglycans / metabolism* Humans Interleukin-1 / pharmacology Isoenzymes / metabolism* Membrane Proteins Phospholipases A / metabolism* Prostaglandin-Endoperoxide Synthases / metabolism* Protein Binding Subcellular Fractions / enzymology Tumor Necrosis Factor-alpha / pharmacology |
| 引用数 | 25 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BRL(RCB0273) |