| RRC ID |
10802
|
| 著者 |
Ikeda K, Mizutani T, Hoshi O, Ushiki T, Haga H, Kawabata K.
|
| タイトル |
Intermediate structure between chromatin fibers and chromosome revealed by mechanical stretching and SPM measurement.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
The morphology of chromosomes (certain rod-shaped structures) is highly reproducible despite the high condensation of chromatin fibers (∼1 mm) into chromosomes (∼1 μm). However, the mechanism underlying the condensation of chromatin fibers into chromosomes is unclear. We assume that investigation of the internal structure of chromosomes will aid in elucidating the condensation process. In order to observe the detailed structure of a chromosome, we stretched a human chromosome by using a micromanipulator and observed its morphology along the stretched region by scanning probe microscopy (SPM). We found that the chromosome consisted of some fibers that were thicker than chromatin fibers. The found fiber was composed of approximately 90-nm-wide beads that were linked linearly. To explore the components of the fiber, we performed immunofluorescence staining of the stretched chromosome. Fluorescence signals of topoisomerase (Topo) IIα, which is known to interact with and support chromatin fibers, and DNA were detected both on the found fiber and beads. Furthermore, after micrococcal nuclease and trypsin treatments, the fibers were found to be mechanically supported by proteins. These results suggest that chromosome comprises an intermediate structure between chromatin fibers and chromosomes.
|
| 巻・号 |
400(1)
|
| ページ |
181-6
|
| 公開日 |
2010-9-10
|
| DOI |
10.1016/j.bbrc.2010.08.041
|
| PII |
S0006-291X(10)01536-6
|
| PMID |
20719238
|
| MeSH |
Chromatin / chemistry*
Chromatin / ultrastructure
Chromosomes, Human / chemistry*
Chromosomes, Human / ultrastructure
Humans
Microscopy, Scanning Probe
Tensile Strength*
|
| IF |
2.985
|
| 引用数 |
0
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
BIOPHYSICS
|
| リソース情報 |
| ヒト・動物細胞 |
BALL-1(RCB0256) |
