RRC ID 10803
著者 Ushiki T, Hoshi O.
タイトル Atomic force microscopy for imaging human metaphase chromosomes.
ジャーナル Chromosome Res
Abstract The present study introduces the principle of atomic force microscopy (AFM) and reviews our results of human metaphase chromosomes obtained by AFM. AFM imaging of the chromosomes revealed that the chromatid arm was not uniform in structure but had ridges and grooves along its length, which was most prominent in the late metaphase. The arrangement of these ridges and grooves was roughly symmetrical with the counterpart of the paired sister chromatids. AFM imaging of banded chromosomes also showed that the ridges and grooves were related to the G/Q-positive and G/Q-negative bands, respectively. At high magnification, the chromatid was seen to be produced by the compaction of highly twisted chromatin fiber loops, and its compaction tended to be stronger in the ridged regions of the chromosomes than in the grooved regions. Our AFM studies also showed the presence of catenation of chromatin fibers between the ridged portions of the chromatid in the late metaphase. Thus, AFM is useful for obtaining the three-dimensional surface topography not only in ambient conditions but also in physiological liquid conditions, and is expected to be an attractive tool for investigating the structure of chromosomes.
巻・号 16(3)
ページ 383-96
公開日 2008-1-1
DOI 10.1007/s10577-008-1241-7
PMID 18461479
MeSH Cell Line Chromatin / ultrastructure Chromosome Banding Chromosomes, Human / ultrastructure* Humans Metaphase Microscopy, Atomic Force / instrumentation Microscopy, Atomic Force / methods* Microscopy, Electron, Scanning
IF 3.413
引用数 25
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 BALL-1(RCB0256)