論文 - 詳細
| RRC ID | 10804 |
|---|---|
| 著者 | Morimoto S, Kurtti TJ, Noda H. |
| タイトル | In vitro cultivation and antibiotic susceptibility of a Cytophaga-like intracellular symbiote isolated from the tick Ixodes scapularis. |
| ジャーナル | Curr Microbiol |
| Abstract |
A Cytophaga-like organism (CLO), isolated from the tick Ixodes scapularis (IsCLO), was adapted to growth in insect cell lines and its antibiotic sensitivity was tested. IsCLO were introduced to four insect cell lines, and their growth was measured by quantitative polymerase chain reaction. IsCLO propagated well in a mosquito cell line, AeAl-2, and caused cytopathic effects in host cells. A lepidopteran cell line, HZ-AM1, was also suitable for propagation of IsCLO and kept a steady state with bacterial growth. Using IsCLO-infected AeAl-2, antibiotics effective against the bacteria included ampicillin, chloramphenicol, penicillin-G, rifampicin, and tetracycline. These antibiotics will be useful for eliminating CLO from host arthropods, which is necessary for in vivo studies of the intracellular facultative symbiotes. |
| 巻・号 | 52(4) |
| ページ | 324-9 |
| 公開日 | 2006-4-1 |
| DOI | 10.1007/s00284-005-0349-7 |
| PMID | 16550461 |
| MeSH | Animals Anti-Bacterial Agents / pharmacology* Cell Line Cytophaga / drug effects Cytophaga / isolation & purification Cytophaga / physiology* Drug Resistance, Bacterial* Insecta / cytology Ixodes / microbiology* Microbial Viability / drug effects Symbiosis |
| IF | 1.746 |
| 引用数 | 24 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIAS-AeAl-2(RCB0277) |