論文 - 詳細
| RRC ID | 1081 |
|---|---|
| 著者 | Endo S, Komori T, Ricci G, Sano A, Yokoyama K, Ohori A, Kamei K, Franco M, Miyaji M, Nishimura K. |
| タイトル | Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
Paracoccidioidomycosis is a deep mycosis caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. The amplified DNA appeared as a ladder with a specific banding pattern. The advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. |
| 巻・号 | 234(1) |
| ページ | 93-7 |
| 公開日 | 2004-5-1 |
| DOI | 10.1016/j.femsle.2004.03.015 |
| PII | S0378109704001995 |
| PMID | 15109725 |
| MeSH | Animals Antigens, Fungal / genetics* Armadillos / microbiology DNA Fingerprinting / methods DNA, Fungal / analysis Fungal Proteins / genetics* Genes, Fungal / genetics Glycoproteins / genetics* Humans Nucleic Acid Amplification Techniques / methods* Paracoccidioides / genetics* Paracoccidioides / isolation & purification Paracoccidioidomycosis / diagnosis Paracoccidioidomycosis / microbiology Paraffin Embedding Polymerase Chain Reaction / methods |
| IF | 1.987 |
| 引用数 | 65 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 病原微生物 | IFM 5740 IFM 5830 IFM 41316 FM 41329 IFM 41620 IFM 41621 IFM 41622 IFM 41623 IFM 41624 IFM 41625 IFM 41626 IFM 41628 IFM 41629 IFM 41630 IFM 41631 IFM 41632 IFM 41633 IFM 41708 IFM 46215 IFM 46240 IFM 46463 IFM 46464 IFM 46465 IFM 46466 IFM 46467 IFM 46468 IFM 46470 IFM 46930 IFM 47068 IFM 47183 IFM 47185 IFM 47195 IFM 47219 IFM 47228 IFM 47247 IFM 50993 |