| RRC ID |
10826
|
| 著者 |
Taira M, Sasaki K, Saitoh S, Nezu T, Sasaki M, Kimura S, Terasaki K, Sera K, Narushima T, Araki Y.
|
| タイトル |
Accumulation of element Ti in macrophage-like RAW264 cells cultured in medium with 1 ppm Ti and effects on cell viability, SOD production and TNF-alpha secretion.
|
| ジャーナル |
Dent Mater J
|
| Abstract |
The adverse effect of Ti on body-defense macrophage is not well understood. The aims of this study were twofold: (1) to examine the intracellular accumulation of Ti element; and (2) to measure the cell viability, superoxide dismutase (SOD) production, and TNF-alpha secretion of macrophage-like RAW264 cells cultured for two days in medium with 1 ppm Ti prepared from acidic ICP Ti standard solution. PIXE analysis showed that element Ti was accumulated up to 7.3 ppm in RAW264 cells when cultured in the medium with 1 ppm Ti. Further, RAW264 cells cultured in the medium with 1 ppm Ti exhibited cell viability of about 60%, SOD production of about 180%, and TNF-alpha secretion of about 170% relative to those of control cells cultured in the medium without Ti. It was speculated that phagocytosis of minute Ti-containing complex (mostly TiO2) by macrophage caused oxidative stress and inflammatory reaction, leading to cell proliferation arrest and increased production of SOD and TNF-alpha.
|
| 巻・号 |
25(4)
|
| ページ |
726-32
|
| 公開日 |
2006-12-1
|
| DOI |
10.4012/dmj.25.726
|
| PMID |
17338307
|
| MeSH |
Animals
Cell Line
Cell Survival / drug effects
Culture Media, Conditioned
Macrophages / drug effects*
Macrophages / metabolism
Mice
Oxidative Stress
Spectrometry, X-Ray Emission
Superoxide Dismutase / biosynthesis*
Titanium / analysis
Titanium / toxicity*
Tumor Necrosis Factor-alpha / metabolism*
|
| IF |
1.359
|
| 引用数 |
13
|
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
