Reference - Detail
| RRC ID | 10829 |
|---|---|
| Author | Fujiyama K, Misaki R, Katsura A, Tanaka T, Furukawa A, Omasa T, Seki T. |
| Title | N-linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells. |
| Journal | J Biosci Bioeng |
| Abstract |
cDNA encoding H- and L-chains from a mouse monoclonal antibody was introduced into tobacco BY2 cells, and the resulting sugar chain structures of plant-produced antibodies were analyzed by a combination of HPLC, exoglucosidase digestion and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The glycan structures determined were Man(5-6)GlcNAc2 (22.3%), GlcNAcMan5GlcNAc2 (3.1%), GlcNAcMan3FucXylGlcNAc2 (24.4%), GlcNAcMan3XylGlcNAc2 (17.8%), Man3FucXylGlcNAc2 (24.3%), and Man3XylGlcNAc2 (8.1%). The major glycan structures of the antibodies produced by transgenic suspension-cultured cells contain typical plant bisecting beta(1,2)-xylose and alpha(1,3)-fucose residues, suggesting the posttranslational modification of a recombinant antibody in the late Golgi apparatus. |
| Volume | 101(3) |
| Pages | 212-8 |
| Published | 2006-3-1 |
| DOI | 10.1263/jbb.101.212 |
| PII | S1389-1723(06)70568-0 |
| PMID | 16716920 |
| MeSH | Animals Antibodies, Monoclonal / chemistry* Base Sequence Carbohydrate Sequence Cloning, Molecular Genes, Plant Glucosidases / metabolism Golgi Apparatus / metabolism Immunoglobulin G / chemistry Mice Molecular Sequence Data Plants / chemistry Plants / metabolism Polysaccharides / chemistry* Tobacco / metabolism* |
| IF | 2.366 |
| Times Cited | 20 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Human and Animal Cells | 3A21(RCB1285) |