論文 - 詳細
RRC ID | 10830 |
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著者 | Saitoh Y, Yoshimura Y, Nakano K, Miwa N. |
タイトル | Platinum nanocolloid-supplemented hydrogendissolved water inhibits growth of human tongue carcinoma cells preferentially over normal cells. |
ジャーナル | Exp Oncol |
Abstract |
AIM:Hydrogen-dissolved water (HD-water) or platinum nanocolloid (Pt-nc) has been individually expected as a new therapeutic agent for oxidative stress-related diseases, whereas little is known about their combined effects on cancer, which were elucidated in the present study. METHODS:HD-water was prepared by microporous gas bubbling, and supplemented with Pt-nc consisting of 0.003-1 ppm Pt and PVP polymers. Antioxidant activities were examined by 1, 1-diphenyl-picrylhydrazyl (DPPH)-radicalscavenging assay. Cytotoxic activities were examined by culturing of tumor and normal cell lines, respectively. RESULTS:HD-water accelerated the Pt-nc-based DPPH-radical scavenging. Pt-nc-supplemented HD-water inhibited either colony formation efficiencies or colony sizes of human tongue carcinoma cells HSC-4, in contrast to no effects of HD-water alone, Pt-nc alone or Pt-absent PVP, but not appreciably inhibit normal human tongue epithelial-like cells DOK. Pt-nc-supplemented HD-water also suppressed cell population growth of HSC-4 cells of near-confluence (at higher cell densities) in view of decreases in either cell numbers or mitochondrial function, although less markedly than colony formation starting from a sparse-cell state (at lower cell densities). Dissolved hydrogen, oxygen concentration or oxido-reduced potentials of HD-water was decreased, rather decreased or increased by Pt-nc addition, respectively. CONCLUSIONS:Anti-cancer activity of Pt-nc-supplemented HD-water was shown by its preferential cell-growth inhibition to human tongue carcinoma cells HSC-4 over normal human tongue cells DOK, and might be partly attributed to HD-water-caused enhancement of Pt-nc-relevant antioxidant ability. Pt-nc-supplemented HD-water is expected as a novel agent against human tongue cancers due to its cancer progression-repressive abilities. |
巻・号 | 31(3) |
ページ | 156-62 |
公開日 | 2009-9-1 |
PII | 40/772 |
PMID | 19783965 |
MeSH | Antineoplastic Agents / pharmacology* Biphenyl Compounds Cell Proliferation / drug effects* Cell Survival / drug effects Colloids / chemistry Colony-Forming Units Assay Free Radical Scavengers Humans Hydrogen / chemistry* Metal Nanoparticles Picrates Platinum / chemistry* Tongue Neoplasms / pathology Tongue Neoplasms / prevention & control* Water / chemistry Water / pharmacology* |
リソース情報 | |
ヒト・動物細胞 | SC-4(RCB1902) |