論文 - 詳細
| RRC ID | 10846 |
|---|---|
| 著者 | Nakajima Y, Yamazaki T, Nishii S, Noguchi T, Hoshino H, Niwa K, Viviani VR, Ohmiya Y. |
| タイトル | Enhanced beetle luciferase for high-resolution bioluminescence imaging. |
| ジャーナル | PLoS One |
| Abstract |
We developed an enhanced green-emitting luciferase (ELuc) to be used as a bioluminescence imaging (BLI) probe. ELuc exhibits a light signal in mammalian cells that is over 10-fold stronger than that of the firefly luciferase (FLuc), which is the most widely used luciferase reporter gene. We showed that ELuc produces a strong light signal in primary cells and tissues and that it enables the visualization of gene expression with high temporal resolution at the single-cell level. Moreover, we successfully imaged the nucleocytoplasmic shuttling of importin alpha by fusing ELuc at the intracellular level. These results demonstrate that the use of ELuc allows a BLI spatiotemporal resolution far greater than that provided by FLuc. |
| 巻・号 | 5(4) |
| ページ | e10011 |
| 公開日 | 2010-4-2 |
| DOI | 10.1371/journal.pone.0010011 |
| PMID | 20368807 |
| PMC | PMC2848861 |
| MeSH | Animals Coleoptera / enzymology Diagnostic Imaging / methods* Humans Karyopherins / analysis Karyopherins / metabolism Luciferases* Luminescent Measurements Luminescent Proteins Molecular Probe Techniques Molecular Probes* Protein Transport Proteins / analysis* |
| IF | 2.74 |
| 引用数 | 78 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3 (RCB1862) |