論文 - 詳細
| RRC ID | 10851 |
|---|---|
| 著者 | Niwa K, Nakajima Y, Ohmiya Y. |
| タイトル | Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase. |
| ジャーナル | Anal Biochem |
| Abstract |
Based on the biosynthetic pathway of firefly bioluminescence substrate D-luciferin, the concentration of L-cysteine can be quantified using a simple protocol and a conventional luminescence detector. The lower limit of quantification (signal/noise ratio [S/N]=10) was 0.26 microM. Using our method, the total amount of free/reduced and disulfide/oxidized L-cysteine could be measured successfully in human serum. In addition, biosynthetic precursors such as 2-cyano-6-hydroxybenzothiazole and L-luciferin could replace d-luciferin in the cell-based luciferase assay. Our results suggest that the bioluminescence reaction associated with the biosynthesis of bioluminescence substrates can provide a fast and cost-effective assay method. |
| 巻・号 | 396(2) |
| ページ | 316-8 |
| 公開日 | 2010-1-15 |
| DOI | 10.1016/j.ab.2009.09.014 |
| PII | S0003-2697(09)00637-X |
| PMID | 19748476 |
| MeSH | Benzothiazoles / chemistry Benzothiazoles / metabolism Cysteine / blood* Cysteine / metabolism Firefly Luciferin / metabolism* Humans Luciferases / metabolism* Luminescent Measurements / methods* Protein Biosynthesis |
| IF | 2.877 |
| 引用数 | 13 |
| WOS 分野 | CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3-3-4(RCB1862) |