RRC ID 10854
Author Inagaki Y, Johzuka-Hisatomi Y, Mori T, Takahashi S, Hayakawa Y, Peyachoknagul S, Ozeki Y, Iida S.
Title Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories.
Journal Gene
Abstract Genomic DNA segments (approximately 17kb) containing three DFR genes in the Japanese and common morning glories were sequenced. The three DFR genes in both plants were found to be arranged in a tandem array, and all of them comprised six exons with identical intron positions. Their DFR-B genes carrying longer introns than the DFR-A and DFR-C genes were expressed extensively in the young buds of pigmented flowers, and the transcription starting site for the DFR-B mRNA of the Japanese morning glory was determined. The DFR-B gene of the common morning glory was expressed considerably in stems, moderately in sepals and leaves, whereas the DFR-A and DFR-C genes of the same plant were expressed scarcely but significantly in the young flower buds and stems. Several novel mobile element-like sequences of around 200bp were found in the genomic DFR regions. A phylogenetic tree indicated that each DFR gene in the Japanese morning glory is most closely related to the corresponding DFR gene in the common morning glory, and that the DFR-B gene is the most diversified gene among the three DFR genes. These structural and functional features of the DFR genes and their evolutionary implications are discussed.
Volume 226(2)
Pages 181-8
Published 1999-1-21
DOI 10.1016/s0378-1119(98)00571-x
PII S037811199800571X
PMID 9931484
MeSH Alcohol Oxidoreductases / genetics* Base Sequence DNA Primers Phylogeny Pigmentation / genetics* Retroelements Sequence Homology, Nucleic Acid Solanaceae / classification Solanaceae / enzymology Solanaceae / genetics*
IF 2.984
Times Cited 57
Morning Glory AK5 AK9 AK303 AK311