RRC ID |
10854
|
著者 |
Inagaki Y, Johzuka-Hisatomi Y, Mori T, Takahashi S, Hayakawa Y, Peyachoknagul S, Ozeki Y, Iida S.
|
タイトル |
Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories.
|
ジャーナル |
Gene
|
Abstract |
Genomic DNA segments (approximately 17kb) containing three DFR genes in the Japanese and common morning glories were sequenced. The three DFR genes in both plants were found to be arranged in a tandem array, and all of them comprised six exons with identical intron positions. Their DFR-B genes carrying longer introns than the DFR-A and DFR-C genes were expressed extensively in the young buds of pigmented flowers, and the transcription starting site for the DFR-B mRNA of the Japanese morning glory was determined. The DFR-B gene of the common morning glory was expressed considerably in stems, moderately in sepals and leaves, whereas the DFR-A and DFR-C genes of the same plant were expressed scarcely but significantly in the young flower buds and stems. Several novel mobile element-like sequences of around 200bp were found in the genomic DFR regions. A phylogenetic tree indicated that each DFR gene in the Japanese morning glory is most closely related to the corresponding DFR gene in the common morning glory, and that the DFR-B gene is the most diversified gene among the three DFR genes. These structural and functional features of the DFR genes and their evolutionary implications are discussed.
|
巻・号 |
226(2)
|
ページ |
181-8
|
公開日 |
1999-1-21
|
DOI |
10.1016/s0378-1119(98)00571-x
|
PII |
S037811199800571X
|
PMID |
9931484
|
MeSH |
Alcohol Oxidoreductases / genetics*
Base Sequence
DNA Primers
Phylogeny
Pigmentation / genetics*
Retroelements
Sequence Homology, Nucleic Acid
Solanaceae / classification
Solanaceae / enzymology
Solanaceae / genetics*
|
IF |
2.984
|
引用数 |
57
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
アサガオ |
AK5
AK9
AK303
AK311 |