論文 - 詳細
| RRC ID | 10882 |
|---|---|
| 著者 | Harada K, Ohba K, Ozaki S, Isse K, Hirayama T, Wada A, Nakanuma Y. |
| タイトル | Peptide antibiotic human beta-defensin-1 and -2 contribute to antimicrobial defense of the intrahepatic biliary tree. |
| ジャーナル | Hepatology |
| Abstract |
Human beta-defensins (hBDs) are important antimicrobial peptides that contribute to innate immunity at mucosal surfaces. This study was undertaken to investigate the expression of hBD-1 and hBD-2 in intrahepatic biliary epithelial cells in specimens of human liver, and 4 cultured cell lines (2 consisting of biliary epithelial cells and 2 cholangiocarcinoma cells). In addition, hBD-1 and hBD-2 were assayed in specimens of bile. hBD-1 was nonspecifically expressed immunohistochemically in intrahepatic biliary epithelium and hepatocytes in all patients studied, but expression of hBD-2 was restricted to large intrahepatic bile ducts in 8 of 10 patients with extrahepatic biliary obstruction (EBO), 7 of 11 with hepatolithiasis, 1 of 6 with primary biliary cirrhosis (PBC), 1 of 5 with primary sclerosing cholangitis (PSC), 0 of 6 with chronic hepatitis C (CH-C), and 0 of 11 with normal hepatic histology. hBD-2 expression was evident in bile ducts exhibiting active inflammation. Serum C reactive protein levels correlated with biliary epithelial expression of hBD-2. Real-time PCR revealed that in all of 28 specimens of fresh liver, including specimens from patients with hepatolithiasis, PBC, PSC, CH-C and normal hepatic histology, hBD-1 messenger RNA was consistently expressed, whereas hBD-2 messenger RNA was selectively expressed in biliary epithelium of patients with hepatolithiasis. Immunobloting analysis revealed hBD-2 protein in bile in 1 of 3 patients with PSC, 1 of 3 with PBC, and each of 6 with hepatolithiasis; in contrast, hBD-1 was detectable in all bile samples examined. Four cultured biliary epithelial cell lines consistently expressed hBD-1; in contrast these cell lines did not express hBD-2 spontaneously but were induced to express hBD-2 by treatment with Eschericia coli, lipopolysaccharide, interleukin-1beta or tumor necrosis factor-alpha. In conclusion, these findings suggest that in the intrahepatic biliary tree, hBD-2 is expressed in response to local infection and/or active inflammation, whereas hBD-1 may constitute a preexisting component of the biliary antimicrobial defense system. |
| 巻・号 | 40(4) |
| ページ | 925-32 |
| 公開日 | 2004-10-1 |
| DOI | 10.1002/hep.20379 |
| PMID | 15382127 |
| MeSH | Adult Aged Bile / metabolism Bile Duct Diseases / metabolism Bile Duct Diseases / physiopathology* Bile Duct Neoplasms Bile Ducts, Intrahepatic / cytology Bile Ducts, Intrahepatic / metabolism* Cell Line, Tumor Cholangiocarcinoma Epithelial Cells / cytology Epithelial Cells / metabolism Female Gene Expression Hepatocytes / cytology Hepatocytes / metabolism Humans In Situ Hybridization Liver Diseases / metabolism Liver Diseases / physiopathology* Male Middle Aged RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction beta-Defensins / genetics* beta-Defensins / metabolism* |
| IF | 14.679 |
| 引用数 | 72 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1648) |