RRC ID 1089
Author Sugita T, Takeo K, Ohkusu M, Virtudazo E, Takashima M, Asako E, Ohshima F, Harada S, Yanaka C, Nishikawa A, Majoros L, Sipiczki M.
Title Fluconazole-resistant pathogens Candida inconspicua and C. norvegensis: DNA sequence diversity of the rRNA intergenic spacer region, antifungal drug susceptibility, and extracellular enzyme production.
Journal Microbiol. Immunol.
Abstract The opportunistic fungal pathogens Candida inconspicua and C. norvegensis are very rarely isolated from patients and are resistant to fluconazole. We collected 38 strains of the two microorganisms isolated from Europe and Japan, and compared the polymorphism of the rRNA intergenic spacer (IGS) and internal transcribed spacer (ITS) regions, antifungal drug susceptibility, and extracellular enzyme production as a potential virulence factor. While the IGS sequences of C. norvegensis were not very divergent (more than 96.7% sequence similarity among the strains), those of C. inconspicua showed remarkable diversity, and were divided into four genotypes with three subtypes. In the ITS region, no variation was found in either species. Since the sequence similarity of the two species is approximately 70% at the ITS region, they are closely related phylogenetically. Fluconazole resistance was reconfirmed for the two microorganisms but they were susceptible to micafungin and amphotericin B. No strain of either species secreted aspartyl proteinase or phospholipase B. These results provide basal information for accurate identification, which is of benefit to global molecular epidemiological studies and facilitates our understanding of the medical mycological characteristics of C. inconspicua and C. norvegensis.
Volume 48(10)
Pages 761-6
Published 2004
PII JST.JSTAGE/mandi/48.761
PMID 15502409
MeSH Amphotericin B / pharmacology Antifungal Agents / pharmacology* Aspartic Acid Endopeptidases / metabolism* Base Sequence Candida / classification Candida / drug effects* Candida / enzymology Candida / genetics DNA, Ribosomal Spacer / analysis* Drug Resistance, Fungal* Echinocandins Fluconazole / pharmacology* Genetic Variation Lipopeptides Lipoproteins / pharmacology Lysophospholipase / metabolism* Micafungin Molecular Sequence Data Peptides, Cyclic / pharmacology RNA, Ribosomal / genetics Virulence / genetics
IF 1.335
Times Cited 11
Pathogenic microorganisms IFM 53506 IFM 53507 IFM 53508 IFM 53509 IFM 53510 IFM 53511 IFM 53512 IFM 53513 IFM 53514 IFM 53515 IFM 53516 IFM 53793 IFM 53794 IFM 53795 IFM 53796 IFM 53797 IFM 53798 IFM 53799 IFM 53800 IFM 53801 IFM 53802 IFM 53803 IFM 53804 IFM 53805 IFM 53806 IFM 53807 IFM 53808 IFM 53809 IFM 53810 IFM 53811 IFM 53812 IFM 53838