| RRC ID |
1089
|
| 著者 |
Sugita T, Takeo K, Ohkusu M, Virtudazo E, Takashima M, Asako E, Ohshima F, Harada S, Yanaka C, Nishikawa A, Majoros L, Sipiczki M.
|
| タイトル |
Fluconazole-resistant pathogens Candida inconspicua and C. norvegensis: DNA sequence diversity of the rRNA intergenic spacer region, antifungal drug susceptibility, and extracellular enzyme production.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
The opportunistic fungal pathogens Candida inconspicua and C. norvegensis are very rarely isolated from patients and are resistant to fluconazole. We collected 38 strains of the two microorganisms isolated from Europe and Japan, and compared the polymorphism of the rRNA intergenic spacer (IGS) and internal transcribed spacer (ITS) regions, antifungal drug susceptibility, and extracellular enzyme production as a potential virulence factor. While the IGS sequences of C. norvegensis were not very divergent (more than 96.7% sequence similarity among the strains), those of C. inconspicua showed remarkable diversity, and were divided into four genotypes with three subtypes. In the ITS region, no variation was found in either species. Since the sequence similarity of the two species is approximately 70% at the ITS region, they are closely related phylogenetically. Fluconazole resistance was reconfirmed for the two microorganisms but they were susceptible to micafungin and amphotericin B. No strain of either species secreted aspartyl proteinase or phospholipase B. These results provide basal information for accurate identification, which is of benefit to global molecular epidemiological studies and facilitates our understanding of the medical mycological characteristics of C. inconspicua and C. norvegensis.
|
| 巻・号 |
48(10)
|
| ページ |
761-6
|
| 公開日 |
2004-1-1
|
| DOI |
10.1111/j.1348-0421.2004.tb03602.x
|
| PII |
JST.JSTAGE/mandi/48.761
|
| PMID |
15502409
|
| MeSH |
Amphotericin B / pharmacology
Antifungal Agents / pharmacology*
Aspartic Acid Endopeptidases / metabolism*
Base Sequence
Candida / classification
Candida / drug effects*
Candida / enzymology
Candida / genetics
DNA, Ribosomal Spacer / analysis*
Drug Resistance, Fungal*
Echinocandins
Fluconazole / pharmacology*
Genetic Variation
Lipopeptides
Lipoproteins / pharmacology
Lysophospholipase / metabolism*
Micafungin
Molecular Sequence Data
Peptides, Cyclic / pharmacology
RNA, Ribosomal / genetics
Virulence / genetics
|
| IF |
1.566
|
| 引用数 |
18
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 53506
IFM 53507
IFM 53508
IFM 53509
IFM 53510
IFM 53511
IFM 53512
IFM 53513
IFM 53514
IFM 53515
IFM 53516
IFM 53793
IFM 53794
IFM 53795
IFM 53796
IFM 53797
IFM 53798
IFM 53799
IFM 53800
IFM 53801
IFM 53802
IFM 53803
IFM 53804
IFM 53805
IFM 53806
IFM 53807
IFM 53808
IFM 53809
IFM 53810
IFM 53811
IFM 53812
IFM 53838 |
