RRC ID 10895
Author Inoue M, Iida A, Satoh S, Kodama T, Watanabe S.
Title COUP-TFI and -TFII nuclear receptors are expressed in amacrine cells and play roles in regulating the differentiation of retinal progenitor cells.
Journal Exp. Eye Res.
Abstract Chicken ovalbumin upstream promoter transcription factors (COUP-TFs) are members of the steroid/thyroid hormone receptor superfamily. We have shown that two homologous COUP-TF genes, COUP-TFI and COUP-TFII, are expressed in developing mouse retina with a unique gradient along the dorsal-ventral axis. In this work, we aimed to characterize the detailed expression patterns of COUP-TFs in mature retina. Their functions in retinal progenitor cell differentiation into subtypes of mature retinal cells were also examined. Immunostaining of frozen mouse retinal sections with antibodies against COUP-TFs and markers for retinal subtypes revealed that COUP-TFI and -TFII are expressed in amacrine cells, especially in a glycinergic subtype in mature mouse retina. Forced expression of COUP-TFI and -TFII in mouse retinal explant culture by retrovirus-mediated gene transfer promoted amacrine and cone photoreceptor cell differentiation, whereas that of rod photoreceptors decreased. Cell proliferation and apoptosis were not affected by the perturbation of COUP-TFI and -TFII expression levels. Using the Y79 retinoblastoma cell line, we observed that COUP-TFI and -TFII suppressed the transcriptional activation of the Nrl gene. We then analyzed one another member of COUP-TF transcription factors, COUP-TFgamma, whose structure is relatively distant from those of COUP-TFI and -TFII. It is expressed mainly in horizontal cells and has weak activity in inducing amacrine cells when COUP-TFgamma was ectopically expressed in retinal explants. In summary, we found that COUP-TFI and -TFII play roles in amacrine cell differentiation, and COUP-TFgamma has distinct expression pattern and roles during retinal development.
Volume 90(1)
Pages 49-56
Published 2010-1
DOI 10.1016/j.exer.2009.09.009
PII S0014-4835(09)00277-2
PMID 19766631
MeSH Amacrine Cells / metabolism* Animals Apoptosis Basic-Leucine Zipper Transcription Factors / genetics COUP Transcription Factor I / genetics* COUP Transcription Factor II / genetics* Cell Differentiation / physiology* Cell Proliferation Eye Proteins / genetics Flow Cytometry Gene Expression Regulation / physiology* Mice Mice, Inbred ICR Plasmids Retina / cytology* Retinal Neoplasms / genetics Retinoblastoma / genetics Reverse Transcriptase Polymerase Chain Reaction Stem Cells / cytology* Transfection Tumor Cells, Cultured
IF 3.152
Times Cited 8
WOS Category OPHTHALMOLOGY
Resource
Human and Animal Cells Y79 (RCB1645)