論文 - 詳細
| RRC ID | 10895 |
|---|---|
| 著者 | Inoue M, Iida A, Satoh S, Kodama T, Watanabe S. |
| タイトル | COUP-TFI and -TFII nuclear receptors are expressed in amacrine cells and play roles in regulating the differentiation of retinal progenitor cells. |
| ジャーナル | Exp Eye Res |
| Abstract |
Chicken ovalbumin upstream promoter transcription factors (COUP-TFs) are members of the steroid/thyroid hormone receptor superfamily. We have shown that two homologous COUP-TF genes, COUP-TFI and COUP-TFII, are expressed in developing mouse retina with a unique gradient along the dorsal-ventral axis. In this work, we aimed to characterize the detailed expression patterns of COUP-TFs in mature retina. Their functions in retinal progenitor cell differentiation into subtypes of mature retinal cells were also examined. Immunostaining of frozen mouse retinal sections with antibodies against COUP-TFs and markers for retinal subtypes revealed that COUP-TFI and -TFII are expressed in amacrine cells, especially in a glycinergic subtype in mature mouse retina. Forced expression of COUP-TFI and -TFII in mouse retinal explant culture by retrovirus-mediated gene transfer promoted amacrine and cone photoreceptor cell differentiation, whereas that of rod photoreceptors decreased. Cell proliferation and apoptosis were not affected by the perturbation of COUP-TFI and -TFII expression levels. Using the Y79 retinoblastoma cell line, we observed that COUP-TFI and -TFII suppressed the transcriptional activation of the Nrl gene. We then analyzed one another member of COUP-TF transcription factors, COUP-TFgamma, whose structure is relatively distant from those of COUP-TFI and -TFII. It is expressed mainly in horizontal cells and has weak activity in inducing amacrine cells when COUP-TFgamma was ectopically expressed in retinal explants. In summary, we found that COUP-TFI and -TFII play roles in amacrine cell differentiation, and COUP-TFgamma has distinct expression pattern and roles during retinal development. |
| 巻・号 | 90(1) |
| ページ | 49-56 |
| 公開日 | 2010-1-1 |
| DOI | 10.1016/j.exer.2009.09.009 |
| PII | S0014-4835(09)00277-2 |
| PMID | 19766631 |
| MeSH | Amacrine Cells / metabolism* Animals Apoptosis Basic-Leucine Zipper Transcription Factors / genetics COUP Transcription Factor I / genetics* COUP Transcription Factor II / genetics* Cell Differentiation / physiology* Cell Proliferation Eye Proteins / genetics Flow Cytometry Gene Expression Regulation / physiology* Mice Mice, Inbred ICR Plasmids Retina / cytology* Retinal Neoplasms / genetics Retinoblastoma / genetics Reverse Transcriptase Polymerase Chain Reaction Stem Cells / cytology* Transfection Tumor Cells, Cultured |
| IF | 3.011 |
| 引用数 | 11 |
| WOS 分野 | OPHTHALMOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Y79(RCB1645) |