| RRC ID |
10898
|
| 著者 |
Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E, Hata R.
|
| タイトル |
Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell transplants. [corrected].
|
| ジャーナル |
Transgenic Res
|
| Abstract |
We reported previously that the forced expression of the chemokine BRAK, also called CXCL14 in head and neck squamous cell carcinoma (HNSCC) cells decreased the rate of tumor formation and size of tumor xenografts compared with mock-vector treated cells in athymic nude mice or in severe combined immunodeficiency mice. This suppression occurred even though the growth rates of these cells were the same under in vitro culture conditions, suggesting that a high expression level of the gene in tumor cells is important for the suppression of tumor establishment in vivo. The aim of this study was to determine whether CXCL14/BRAK transgenic mice show resistance to tumor cell xenografts or not. CXCL14/BRAK cDNA was introduced into male C57BL/6 J pronuclei, and 10 founder transgenic mice (Tg) were obtained. Two lines of mice expressed over 10 times higher CXCL14/BRAK protein levels (14 and 11 ng/ml plasma, respectively) than normal blood level (0.9 ng/ml plasma), without apparent abnormality. The sizes of Lewis lung carcinoma and B16 melanoma cell xenografts in Tg mice were significantly smaller than those in control wild-type mice, indicating that CXCL14/BRAK, first found as a suppressor of tumor progression of HNSCC, also suppresses the progression of a carcinoma of other tissue origin. Immunohistochemical studies showed that invasion of blood vessels into tumors was suppressed in tumor xenografts of CXCL14/BRAK Tg mice. These results indicate that CXCL14/BRAK suppressed tumor cell xenografts by functioning paracrine or endocrine fashion and that CXCL14/BRAK is a very promising molecular target for tumor suppression without side effects.
|
| 巻・号 |
19(6)
|
| ページ |
1109-17
|
| 公開日 |
2010-12-1
|
| DOI |
10.1007/s11248-010-9384-7
|
| PMID |
20333465
|
| MeSH |
Animals
Base Sequence
Carcinoma, Lewis Lung / blood supply
Carcinoma, Lewis Lung / genetics
Carcinoma, Lewis Lung / immunology
Carcinoma, Lewis Lung / therapy
Cell Line, Tumor
Chemokines, CXC / genetics*
Chemokines, CXC / immunology*
DNA Primers / genetics
Female
Gene Expression
Humans
Kidney / immunology
Male
Melanoma, Experimental / blood supply
Melanoma, Experimental / genetics
Melanoma, Experimental / immunology
Melanoma, Experimental / therapy
Mice
Mice, Inbred C57BL
Mice, Transgenic
Neoplasm Transplantation
Neoplasms, Experimental / blood supply
Neoplasms, Experimental / genetics
Neoplasms, Experimental / immunology*
Neoplasms, Experimental / therapy*
RNA, Messenger / genetics
Recombinant Proteins / genetics
Recombinant Proteins / immunology
Transplantation, Heterologous
|
| IF |
1.856
|
| 引用数 |
18
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
LLC(RCB0558)
B16 melanoma(RCB1283) |
