RRC ID 10903
著者 Sun L, Fu BB, Liu DG.
タイトル Systemic delivery of full-length C/EBP beta/liposome complex suppresses growth of human colon cancer in nude mice.
ジャーナル Cell Res
Abstract C/EBP beta (CCAAT/enhancer-binding protein beta) is an important transcription factor involved in cellular proliferation and differentiation. Overexpression of the full-length C/EBP beta protein results in cellular growth arrest and apoptosis. Using a nonviral liposome as carrier, we delivered the full-length C/EBP beta expression plasmid, pCN, into nude mice bearing CW-2 human colon cancer tumors via tail vein. Southern blots revealed that the major organs and tumors were transfected. Experimental gene therapy showed that a strong suppression of tumor growth was observed in the pCN-treated mice, and such suppression was due to the overexpression of C/EBP beta, leading to the increased apoptosis in tumors of pCN-treated mice. No apparent toxic effects of pCN/liposome complex were observed in the animals. Thus, C/EBP beta has tumor suppression effect in vivo and may be used in gene therapy for cancers.
巻・号 15(10)
ページ 770-6
公開日 2005-10-1
DOI 10.1038/sj.cr.7290346
PMID 16246266
MeSH Animals CCAAT-Enhancer-Binding Protein-beta / analysis CCAAT-Enhancer-Binding Protein-beta / genetics* Cell Line, Tumor Cell Proliferation Colonic Neoplasms / pathology Colonic Neoplasms / therapy* DNA / analysis Genetic Therapy* Genetic Vectors / toxicity Humans Injections, Intravenous Liposomes / administration & dosage Liposomes / toxicity Mice Mice, Nude
IF 20.507
引用数 15
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 CW-2(RCB0778)