Reference - Detail
| RRC ID | 10909 |
|---|---|
| Author | Munaka T, Abe H, Kanai M, Sakamoto T, Nakanishi H, Yamaoka T, Shoji S, Murakami A. |
| Title | Real-time monitoring of antibody secretion from hybridomas on a microchip by time-resolved luminescence anisotropy analysis. |
| Journal | Anal Biochem |
| Abstract |
This article presents a real-time monitoring system for cellular analysis using micro total analysis systems technology. Time-resolved luminescence anisotropy analysis was adopted for real-time detection of small amounts of a target protein produced by a small number of cells. The system was tested by real-time monitoring of the antibody secretion by hybridomas. The cells were successfully cultivated in a micro-incubation chamber (240 nl) fabricated on a microchip. The quantification of the antibody was achieved using the Ru(II) complex-labeled Staphylococcus aureus protein A probe, which can bind specifically to the Fc region of the antibody. Using this system, we detected as little as 24 fmol of immunoglobulin G under physiological conditions without the bound/free separation protocol. We successfully achieved real-time and quantitative monitoring of small amounts of antibody production by approximately 200 hybridoma cells. This method could be applied to various cellular analyses using small numbers of cells. |
| Volume | 353(1) |
| Pages | 1-6 |
| Published | 2006-6-1 |
| DOI | 10.1016/j.ab.2006.03.009 |
| PII | S0003-2697(06)00174-6 |
| PMID | 16620756 |
| MeSH | Animals Anisotropy Antibody-Producing Cells Equipment Design Hybridomas / chemistry Hybridomas / cytology Hybridomas / metabolism* Immunoglobulin G / analysis* Immunoglobulin G / metabolism Indicators and Reagents Lab-On-A-Chip Devices Luminescent Measurements / methods* Mice Microchip Analytical Procedures / methods* Rats Ruthenium / chemistry Time Factors |
| IF | 2.877 |
| Times Cited | 6 |
| WOS Category | CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 12-1B6(RCB0870) |