Reference - Detail
| RRC ID | 10927 |
|---|---|
| Author | Vazquez G, Lievremont JP, St J Bird G, Putney JW Jr. |
| Title | Human Trp3 forms both inositol trisphosphate receptor-dependent and receptor-independent store-operated cation channels in DT40 avian B lymphocytes. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Mammalian Trp proteins are candidates for plasma membrane calcium channels regulated by receptor activation or by intracellular calcium store depletion [capacitative calcium entry (CCE)]. One extensively investigated member of the Trp family, the human Trp3 (hTrp3), behaves as a receptor-activated, calcium-permeable, nonselective cation channel when expressed in cell lines and does not appear to be activated by store depletion. Nonetheless, there is good evidence that Trp3 can be regulated by interacting with inositol trisphosphate receptors (IP(3)Rs), reminiscent of the conformational coupling mode of CCE. To investigate the role of Trp3 in CCE, and its regulation by IP(3)R, we transiently expressed hTrp3 in the wild-type DT40 chicken B lymphocyte cell line and its variant lacking IP(3)R. Expression of hTrp3 in either wild-type or IP(3)R-knockout cells did not increase basal membrane permeability, but resulted in a substantially greater divalent cation entry after thapsigargin-induced store depletion. This hTrp3-dependent divalent cation entry was significantly greater in the wild type than in IP(3)R-knockout cells. Thus, it appears that in this cell line, hTrp3 forms channels that are store-operated by both IP(3)R-dependent and IP(3)R-independent mechanisms. Trp3, or one of its structural relatives, is a candidate for the store-operated, nonselective cation channels observed in smooth muscle cells and other cell types. |
| Volume | 98(20) |
| Pages | 11777-82 |
| Published | 2001-9-25 |
| DOI | 10.1073/pnas.201238198 |
| PII | 201238198 |
| PMID | 11553786 |
| PMC | PMC58806 |
| MeSH | Animals B-Lymphocytes / drug effects B-Lymphocytes / physiology* Barium / metabolism Calcium / metabolism Calcium Channels / genetics Calcium Channels / physiology* Cell Line Chickens Cloning, Molecular DNA Primers Fura-2 Genetic Vectors Humans Inositol 1,4,5-Trisphosphate Receptors Ion Channels / physiology* Receptors, Cytoplasmic and Nuclear / physiology* Reverse Transcriptase Polymerase Chain Reaction TRPC Cation Channels Thapsigargin / pharmacology Transcription, Genetic Transfection |
| IF | 9.412 |
| Times Cited | 137 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | DT40(RCB1464) IP3R^(-) DT40(RCB1467) |