論文 - 詳細
| RRC ID | 10932 |
|---|---|
| 著者 | Iwashita H, Yoshida M, Nishi T, Otani M, Ueda S. |
| タイトル | In vivo transfer of a neuronal nitric oxide synthase expression vector into the rat bladder by electroporation. |
| ジャーナル | BJU Int |
| Abstract |
OBJECTIVE:To investigate the possibility of in vivo gene transfer by attempting to transfer the neuronal nitric oxide synthase (nNOS) gene into rat bladder using electroporation. MATERIALS AND METHODS:The bladder was exposed through an abdominal midline incision in 8-week-old male rats. Plasmid DNA of the marker genes green fluorescent protein (GFP) and luciferase, and the nNOS gene, was then injected into the subserosal space of the bladder and electroporation applied. At 72 h after gene transfer, GFP and luciferase were assayed in the isolated bladder and immunohistochemical staining used to detect nNOS; NO(x) released from isolated bladder strips was also assessed using microdialysis and high-performance liquid chromatography. RESULTS:From the luciferase assay, 45 V, 1 Hz, 50 ms and eight pulses were selected as the optimum conditions for electroporation. Bladder specimens with GFP genes injected by electroporation showed bright and numerous sites of GFP expression in the smooth muscle layer. In rats with the nNOS gene injected by electroporation there was marked nNOS immunoreactivity, and NO(x) released from bladder strips was significantly greater than in the control groups. CONCLUSIONS:These results suggest that electroporation is a useful technique for in vivo gene transfer into rat bladder smooth muscles, and that the nNOS gene transferred by this procedure functionally expresses and contributes to NO production. |
| 巻・号 | 93(7) |
| ページ | 1098-103 |
| 公開日 | 2004-5-1 |
| DOI | 10.1111/j.1464-410X.2003.04788.x |
| PII | BJU4788 |
| PMID | 15142171 |
| MeSH | Animals DNA, Complementary / metabolism Electroporation / methods* Gene Expression Gene Transfer Techniques Green Fluorescent Proteins Luminescent Proteins Male Muscle Contraction Muscle, Smooth Nitric Oxide Synthase / genetics* Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type I Rats Urinary Bladder / enzymology* |
| IF | 4.806 |
| 引用数 | 8 |
| WOS 分野 | UROLOGY & NEPHROLOGY |
| リソース情報 | |
| ヒト・動物細胞 | DT40(RCB1464) |