論文 - 詳細
| RRC ID | 10963 |
|---|---|
| 著者 | Sakai S, Matsuyama T, Hirose K, Kawakami K. |
| タイトル | In situ simultaneous protein-polysaccharide bioconjugation and hydrogelation using horseradish peroxidase. |
| ジャーナル | Biomacromolecules |
| Abstract |
We propose the peroxidase-catalyzed simultaneous conjugation and hydrogelation of polysaccharide and protein derivatives, each possessing phenolic hydroxyl (Ph) moieties, as a novel route for obtaining protein-polysaccharide conjugate hydrogels. We used alginate, gelatin, and albumin derivatives bearing Ph moieties (Alg-Ph, Gela-Ph, and Alb-Ph) to demonstrate the feasibility. The gelation time of conjugate gels decreased with decreasing H(2)O(2) concentration and with increasing horseradish peroxidase concentration. Gelation time was controllable from a few seconds to 6 min. The repulsion force detected at 40% compression of a conjugate gel obtained from a mixture of Alg-Ph and Gela-Ph at 1.0% (w/v), respectively, was more than 2.8 times larger than that detected for gels produced from 3.0% (w/v) Gela-Ph or 2.0% (w/v) Alg-Ph alone. Cell adhesiveness of gels was tunable by changing the type of protein derivative. A gel from Gela-Ph and Alg-Ph showed higher cell adhesiveness than Alg-Ph gel, but a gel produced from Alb-Ph and Alg-Ph showed a lower cell adhesiveness than Alg-Ph gel. The conjugate gel was degradable by degrading alginate molecules using the nonproteolytic enzyme alginate lyase. The tunable gelation, mechanical properties, and cell adhesiveness of polysaccharide-protein conjugate hydrogels obtained through peroxidase-catalyzed gelation indicates great potential for a wide range of applications, such as scaffolds for tissue engineering and carriers for drug delivery system. |
| 巻・号 | 11(5) |
| ページ | 1370-5 |
| 公開日 | 2010-5-10 |
| DOI | 10.1021/bm1001608 |
| PMID | 20402496 |
| MeSH | Animals Cell Adhesion Cell Line Cell Proliferation Horseradish Peroxidase / chemistry* Hydrogels* Polysaccharides / chemistry* Proteins / chemistry* |
| IF | 6.092 |
| 引用数 | 32 |
| WOS 分野 | POLYMER SCIENCE CHEMISTRY, ORGANIC BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | L929(RCB1451) |