RRC ID 10966
Author Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M.
Title Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT.
Journal J Mol Cell Biol
Abstract We previously established a novel cell line, termed HOZOT, derived from umbilical cord blood mononuclear cells that is characterized as a human cytotoxic regulatory T (Treg) cell line with a FOXP3(+)CD4(+)CD8(+)CD25(+) phenotype. Here, we describe a new property of HOZOT cells: they actively penetrate into a variety of human cancer cell lines, but not into normal cell lines, and form apparent cell-in-cell structures. In the process of cell penetration, we observed that HOZOT cells adhered to target cells seemed to first insert their nuclei into the cytoplasm of target cells, distinct from the process of phagocytosis. In addition, blocking experiments showed that major histocompatibility complex class I is one of the target cell recognition molecules for HOZOT cells. Furthermore, we propose that cell-in-cell structures between HOZOT cells and target cancer cells could be one of the cytotoxic mechanisms of HOZOT cells.
Volume 2(3)
Pages 139-51
Published 2010-6-1
DOI 10.1093/jmcb/mjq002
PII mjq002
PMID 20348186
MeSH Caspase 3 / metabolism Cell Line, Tumor Cytotoxicity, Immunologic* Forkhead Transcription Factors / metabolism Humans Interleukin-2 Receptor alpha Subunit / metabolism Neoplasms / immunology* Phagocytosis T-Lymphocytes, Regulatory / cytology T-Lymphocytes, Regulatory / immunology* T-Lymphocytes, Regulatory / ultrastructure
IF 4.0
Times Cited 18
Human and Animal Cells MKN45(RCB1001)