論文 - 詳細
| RRC ID | 10975 |
|---|---|
| 著者 | Lee KH, Choi EM. |
| タイトル | Biochanin A stimulates osteoblastic differentiation and inhibits hydrogen peroxide-induced production of inflammatory mediators in MC3T3-E1 cells. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Phytoestrogens are plant chemicals that are structurally analogous to estrogen and are known to affect estrogenic activity. Biochanin A, a naturally occurring isoflavone, has been identified and detected in various diets and plant species. We examined the effects of biochanin A on the differentiation of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts. Biochanin A (1-50 microM) caused a significant elevation of cell growth, alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in osteoblastic MC3T3-E1 cells (p<0.05). The effect of biochanin A (10 microM) in increasing ALP activity and collagen content was completely prevented by the presence of 10(-6) M cycloheximide and 10(-6) M tamoxifen, suggesting that biochanin A's effect results from a newly synthesized protein component and might be partly involved in estrogen action. We then examined the effect of biochanin A on the H2O2-induced production of inflammatory mediators in osteoblasts. Biochanin A (1-10 microM) decreased the 0.2 mM H2O2-induced production of TNF-alpha, IL-6 and NO in osteoblasts. These results suggest that biochanin A may be useful as potential phytoestrogens, which play important physiological roles in the prevention of postmenopausal osteoporosis. |
| 巻・号 | 28(10) |
| ページ | 1948-53 |
| 公開日 | 2005-10-1 |
| DOI | 10.1248/bpb.28.1948 |
| PII | JST.JSTAGE/bpb/28.1948 |
| PMID | 16204952 |
| MeSH | 3T3 Cells Alkaline Phosphatase / metabolism Animals Cell Differentiation / drug effects* Genistein / pharmacology* Hydrogen Peroxide / antagonists & inhibitors* Hydrogen Peroxide / pharmacology Inflammation Mediators / metabolism* Mice Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / enzymology Osteoblasts / metabolism |
| IF | 1.863 |
| 引用数 | 33 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |