RRC ID 10975
Author Lee KH, Choi EM.
Title Biochanin A stimulates osteoblastic differentiation and inhibits hydrogen peroxide-induced production of inflammatory mediators in MC3T3-E1 cells.
Journal Biol. Pharm. Bull.
Abstract Phytoestrogens are plant chemicals that are structurally analogous to estrogen and are known to affect estrogenic activity. Biochanin A, a naturally occurring isoflavone, has been identified and detected in various diets and plant species. We examined the effects of biochanin A on the differentiation of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts. Biochanin A (1-50 microM) caused a significant elevation of cell growth, alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in osteoblastic MC3T3-E1 cells (p<0.05). The effect of biochanin A (10 microM) in increasing ALP activity and collagen content was completely prevented by the presence of 10(-6) M cycloheximide and 10(-6) M tamoxifen, suggesting that biochanin A's effect results from a newly synthesized protein component and might be partly involved in estrogen action. We then examined the effect of biochanin A on the H2O2-induced production of inflammatory mediators in osteoblasts. Biochanin A (1-10 microM) decreased the 0.2 mM H2O2-induced production of TNF-alpha, IL-6 and NO in osteoblasts. These results suggest that biochanin A may be useful as potential phytoestrogens, which play important physiological roles in the prevention of postmenopausal osteoporosis.
Volume 28(10)
Pages 1948-53
Published 2005-10
DOI 10.1248/bpb.28.1948
PII JST.JSTAGE/bpb/28.1948
PMID 16204952
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Cell Differentiation / drug effects* Genistein / pharmacology* Hydrogen Peroxide / antagonists & inhibitors* Hydrogen Peroxide / pharmacology Inflammation Mediators / metabolism* Mice Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / enzymology Osteoblasts / metabolism
IF 1.694
Times Cited 26
WOS Category PHARMACOLOGY & PHARMACY
Resource
Human and Animal Cells MC3T3-E1 (RCB1126)