RRC ID 11003
著者 Cuong NX, Tuan TA, Kiem PV, Minh CV, Choi EM, Kim YH.
タイトル New cembranoid diterpenes from the Vietnamese soft coral Sarcophyton mililatensis stimulate osteoblastic differentiation in MC3T3-E1 cells.
ジャーナル Chem Pharm Bull (Tokyo)
Abstract Chemical investigation of the Vietnamese soft coral Sarcophyton mililatensis resulted in the isolation of four new cembranoid diterpenes, namely (-)-7beta-hydroxy-8alpha-methoxydeepoxysarcophytoxide (1), (+)-7beta,8beta-dihydroxydeepoxysarcophytoxide (2), (-)-17-hydroxysarcophytonin A (3) and sarcophytol V (4), along with two known compounds, (+)-sarcophine (5) and sarcophytoxide (6). The NMR spectral data of the new compounds were completely assigned by using a combination of 2D NMR experiments including (1)H-(1)H COSY, HSQC, HMBC, and ROESY. To investigate the bioactivities of compounds, which act on bone metabolism, we studied the effects of compounds on the function of osteoblastic MC3T3-E1 cells. Compound 1 caused a significant elevation of alkaline phosphatase activity, collagen content, and nodules mineralization compared to those of the control (p<0.05). These results suggest that newly isolated compound 1 has a direct stimulatory effect on bone formation in vitro and may contribute to the prevention for osteoporosis.
巻・号 56(7)
ページ 988-92
公開日 2008-7-1
DOI 10.1248/cpb.56.988
PII JST.JSTAGE/cpb/56.988
PMID 18591816
MeSH Alkaline Phosphatase / metabolism Animals Anthozoa / chemistry* Cell Differentiation / drug effects Cell Line Collagen / biosynthesis Diterpenes / chemistry Diterpenes / pharmacology* Magnetic Resonance Spectroscopy Mice Mice, Inbred C57BL Osteoblasts / cytology Osteoblasts / drug effects*
IF 1.416
引用数 28
WOS 分野 CHEMISTRY, MEDICINAL PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)