RRC ID 11035
著者 Omasa T, Furuichi K, Iemura T, Katakura Y, Kishimoto M, Suga K.
タイトル Enhanced antibody production following intermediate addition based on flux analysis in mammalian cell continuous culture.
ジャーナル Bioprocess Biosyst Eng
Abstract Generally, mammalian cells utilize glucose and glutamine as primary energy sources. To investigate the effect of energy sources on metabolic fluxes and antibody production, glucose- or glutamine-limited serum-free continuous culture of hybridoma 3A21 cells, which produce anti-ribonuclease A antibody, was carried out. The cell volume and dry cell weight were evaluated under various steady-state conditions. The specific consumption and production rates were evaluated on the basis of dry cell weight. On the basis of these results, the fluxes of the metabolic pathway were calculated. It was found that increasing the specific growth rate causes the specific ATP and antibody production rates to decrease. The fluxes between malate and pyruvate also decreased with the increase in specific growth rate. To increase the ATP production rate under steady-state conditions by the enhancement of fluxes between malate and pyruvate, the reduced metabolic fluxes were increased by an intermediate (pyruvate, malate, and citrate) addition. As a result, higher specific ATP and antibody production rates were achieved following the intermediate addition at a constant dilution rate.
巻・号 33(1)
ページ 117-25
公開日 2010-1-1
DOI 10.1007/s00449-009-0351-8
PMID 19590901
MeSH Animals Antibodies, Monoclonal / biosynthesis* Cell Culture Techniques / methods* Cell Survival Culture Media, Serum-Free / pharmacology Hybridomas / cytology Hybridomas / metabolism* Mice Models, Biological*
IF 2.419
引用数 22
WOS 分野 ENGINEERING, CHEMICAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ヒト・動物細胞 3A21(RCB1285)