RRC ID 1104
Author Jang MH, Kweon MN, Iwatani K, Yamamoto M, Terahara K, Sasakawa C, Suzuki T, Nochi T, Yokota Y, Rennert PD, Hiroi T, Tamagawa H, Iijima H, Kunisawa J, Yuki Y, Kiyono H.
Title Intestinal villous M cells: an antigen entry site in the mucosal epithelium.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract M cells located in the follicle-associated epithelium of Peyer's patches (PP) are shown to be the principal sites for the sampling of gut luminal antigens. Thus, PP have long been considered the gatekeepers of the mucosal immune system. Here, we report a distinct gateway for the uptake of gut bacteria: clusters of non-follicle-associated epithelium-associated Ulex europaeus agglutinin (UEA)-1(+) cells, which we have designated intestinal villous M cells. Interestingly, villous M cells are developed in various PP [or gut-associated lymphoid tissue (GALT)]-null mice, such as in utero lymphotoxin beta receptor (LTbetaR)-Ig-treated, lymphotoxin alpha (LTalpha)(-/-), tumor necrosis factor/LTalpha(-/-), and inhibition of differentiation 2 (Id2)(-/-) mice. Intestinal villous M cells have been observed to take up GFP-expressing Salmonella, Yersinia, and Escherichia coli-expressing invasin, as well as gut bacterial antigen for subsequent induction of antigen-specific immune responses. Thus, the identified villous M cells could be an alternative and PP-independent gateway for the induction of antigen-specific immune responses by means of the mucosal compartment.
Volume 101(16)
Pages 6110-5
Published 2004-4-20
DOI 10.1073/pnas.0400969101
PII 0400969101
PMID 15071180
PMC PMC395931
MeSH Animals Antigens / immunology* Bacterial Adhesion Intestinal Mucosa / cytology* Intestinal Mucosa / immunology Intestinal Mucosa / microbiology Intestinal Mucosa / ultrastructure Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Microscopy, Electron Peyer's Patches / immunology Salmonella typhimurium / physiology
IF 9.504
Times Cited 277
Pathogenic microorganisms ?