論文 - 詳細
| RRC ID | 11047 |
|---|---|
| 著者 | Ujita M, Yamanaka M, Maeno Y, Yoshida K, Ohshio W, Ueno Y, Banno Y, Fujii H, Okumura H. |
| タイトル | Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase. |
| 巻・号 | 111(1) |
| ページ | 22-5 |
| 公開日 | 2011-1-1 |
| DOI | 10.1016/j.jbiosc.2010.08.020 |
| PII | S1389-1723(10)00289-6 |
| PMID | 20870456 |
| MeSH | Animals Baculoviridae / genetics* Bombyx / genetics Bombyx / metabolism* Bombyx / virology Cloning, Molecular Gene Expression Genetic Vectors Histidine Oligopeptides Recombinant Fusion Proteins / biosynthesis Recombinant Fusion Proteins / genetics Trehalase / biosynthesis* Trehalase / genetics |
| IF | 2.366 |
| 引用数 | 6 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| リソース情報 | |
| カイコ | NA |