RRC ID 1105
著者 Huang X, Phung le V, Dejsirilert S, Tishyadhigama P, Li Y, Liu H, Hirose K, Kawamura Y, Ezaki T.
タイトル Cloning and characterization of the gene encoding the z66 antigen of Salmonella enterica serovar Typhi.
ジャーナル FEMS Microbiol Lett
Abstract Z66 antigen-positive strains of Salmonella enterica serovar Typhi change flagellin expression in only one direction from the z66 antigen to the d or j antigen, which is different from the phase variation of S. enterica serovar Typhimurium. In the present study, we identified a new flagellin gene in z66 antigen-positive strains of S. enterica serovar Typhi. The genomic structure of the region containing this new flagellin gene was similar to that of fljBA operon of biphasic S. enterica serovars. A fljA-like gene was present downstream of the new flagellin gene. A rho-independent terminator was located between the new flagellin gene and the fljA-like gene. Hin-like gene was not present upstream of the new flagellin gene. We generated a mutant strain of S. enterica serovar Typhi, which carries a deletion of the new flagellin gene. Western blotting revealed that the 51-kDa z66 antigen protein was absent from the population of proteins secreted by the mutant strain. Southern hybridization demonstrated that the z66 antigen-positive strains of S. enterica serovar Typhi carried the new flagellin gene and fliC on two different genomic EcoRI fragments. When z66 antigen-positive strains were incubated with anti-z66 antiserum, the flagellin expression by S. enterica serovar Typhi changed from z66 antigen to j antigen. The new flagellin gene and the fljA-like gene were absent in the strain with altered flagellin expression. These results suggested that the new flagellin gene is a fljB-like gene, which encodes the z66 antigen of S. enterica serovar Typhi, and that deletion of fljBA-like operon may explain why S. enterica serovar Typhi alters the flagellin expression in only one direction from the z66 antigen to the d or j antigen.
巻・号 234(2)
ページ 239-46
公開日 2004-5-15
DOI 10.1016/j.femsle.2004.03.030
PII S0378109704002277
PMID 15135528
MeSH Antigens, Bacterial / genetics* Base Sequence Cloning, Molecular DNA Primers Flagellin / genetics Genes, Bacterial* Phylogeny Salmonella typhi / classification Salmonella typhi / genetics* Salmonella typhimurium / classification Salmonella typhimurium / genetics
IF 1.987
引用数 44
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 IID 614(GIFU10007)