RRC ID 11052
著者 Tabunoki H, Ode H, Banno Y, Katsuma S, Shimada T, Mita K, Yamamoto K, Sato R, Ishii-Nozawa R, Satoh J.
タイトル BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
ジャーナル PLoS One
Abstract We cloned cDNA for the Bombyx mori DJ-1 protein (BmDJ-1) from the brains of larvae. BmDJ-1 is composed of 190 amino acids and encoded by 672 nucleotides. Northern blot analysis showed that BmDJ-1 is transcribed as a 756-bp mRNA and has one isoform. Reverse transcriptase (RT)-PCR experiments revealed that the BmDJ-1 was present in the brain, fatbody, Malpighian tubule, ovary and testis but present in only low amounts in the silkgland and hemocyte of day 4 fifth instar larvae. Immunological analysis demonstrated the presence of BmDJ-1 in the brain, midgut, fatbody, Malpighian tubule, testis and ovary from the larvae to the adult. We found that BmDJ-1 has a unique expression pattern through the fifth instar larval to adult developmental stage. We assessed the anti-oxidative function of BmDJ-1 using rotenone (ROT) in day 3 fifth instar larvae. Administration of ROT to day 3 fifth instar larvae, together with exogenous (BmNPV-BmDJ-1 infection for 4 days in advance) BmDJ-1, produced significantly lower 24-h mortality in BmDJ-1 groups than in the control. 2D-PAGE revealed an isoelectric point (pI) shift to an acidic form for BmDJ-1 in BmN4 cells upon ROT stimulus. Among the factors examined for their effects on expression level of BmDJ-1 in the hemolymph, nitric oxide (NO) concentration was identified based on dramatic developmental stage-dependent changes. Administration of isosorbide dinitrate (ISDN), which is an NO donor, to BmN4 cells produced increased expression of BmDJ-1 compared to the control. These results suggest that BmDJ-1 might control oxidative stress in the cell due to NO and serves as a development modulation factor in B. mori.
巻・号 6(3)
ページ e17683
公開日 2011-3-24
DOI 10.1371/journal.pone.0017683
PMID 21455296
PMC PMC3063780
MeSH Animals Antioxidants / metabolism* Bombyx / drug effects Bombyx / genetics Bombyx / metabolism* Hemolymph / drug effects Hemolymph / metabolism Insect Proteins / genetics Insect Proteins / metabolism* Reverse Transcriptase Polymerase Chain Reaction Rotenone / pharmacology
IF 2.74
引用数 5
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ cDNA clone(NRPG1136)