RRC ID 11091
著者 Fan Y, Schlierf M, Gaspar AC, Dreux C, Kpebe A, Chaney L, Mathieu A, Hitte C, Grémy O, Sarot E, Horn M, Zhao Y, Kinzy TG, Rabinow L.
タイトル Drosophila translational elongation factor-1gamma is modified in response to DOA kinase activity and is essential for cellular viability.
ジャーナル Genetics
Abstract Drosophila translational elongation factor-1gamma (EF1gamma) interacts in the yeast two-hybrid system with DOA, the LAMMER protein kinase of Drosophila. Analysis of mutant EF1gamma alleles reveals that the locus encodes a structurally conserved protein essential for both organismal and cellular survival. Although no genetic interactions were detected in combinations with mutations in EF1alpha, an EF1gamma allele enhanced mutant phenotypes of Doa alleles. A predicted LAMMER kinase phosphorylation site conserved near the C terminus of all EF1gamma orthologs is a phosphorylation site in vitro for both Drosophila DOA and tobacco PK12 LAMMER kinases. EF1gamma protein derived from Doa mutant flies migrates with altered mobility on SDS gels, consistent with it being an in vivo substrate of DOA kinase. However, the aberrant mobility appears to be due to a secondary protein modification, since the mobility of EF1gamma protein obtained from wild-type Drosophila is unaltered following treatment with several nonspecific phosphatases. Expression of a construct expressing a serine-to-alanine substitution in the LAMMER kinase phosphorylation site into the fly germline rescued null EF1gamma alleles but at reduced efficiency compared to a wild-type construct. Our data suggest that EF1gamma functions in vital cellular processes in addition to translational elongation and is a LAMMER kinase substrate in vivo.
巻・号 184(1)
ページ 141-54
公開日 2010-1-1
DOI 10.1534/genetics.109.109553
PII genetics.109.109553
PMID 19841092
PMC PMC2815912
MeSH Animals Cell Survival Conserved Sequence Drosophila Proteins / chemistry Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / embryology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Female Gene Expression Regulation, Developmental Genes, Lethal / genetics Humans Larva / growth & development Male Movement Peptide Elongation Factor 1 / chemistry Peptide Elongation Factor 1 / genetics Peptide Elongation Factor 1 / metabolism* Phosphorylation Protein Kinases / metabolism Protein Serine-Threonine Kinases / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Alignment Transcription Factors / metabolism Transcription, Genetic Transgenes / genetics
IF 4.015
引用数 13
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ 11901R-2 11901R-3