論文 - 詳細
| RRC ID | 111 |
|---|---|
| 著者 | Sakuma Y, Sato Y, Inoue S, Kaneko T, Hakamata Y, Takahashi M, Murakami T, Kobayashi E. |
| タイトル | Lympho-myeloid chimerism achieved by spleen graft of green fluorescent protein transgenic rat in a combined pancreas transplantation model (TI03-029). |
| ジャーナル | Transpl Immunol |
| Abstract |
BACKGROUND:Multilineage chimerism is a promising strategy to induce donor-specific tolerance. Because the beneficial effect of splenic grafting on tolerance induction is well known, we studied long-term hematopoietic chimerism and the fate of donor-derived cells after allogenic pancreas/spleen transplantation. METHODS:Green fluorescent protein (GFP) transgenic (Tg) Wistar rats were donors and combined pancreas/spleen transplantation (PST) or pancreas transplantation (PT) alone was performed on recipient LEW rats. Graft survival was compared between these two groups and the fate of donor-derived GFP(+) cells was analyzed by flow cytometry. In this system, the donor-derived cells were clearly defined as having lymphocytic or granulocytic lineage by cell size. T-cell subsets of GFP(+) and GFP(-) cells in long graft-surviving rats were also characterized. RESULTS:The survival period of the grafted pancreas in PST rats was significantly longer than that of PT rats (P<0.001). Three of seven PST rats survived >250 days. The chimeric level of donor-derived GFP(+) cells in the recipient peripheral blood was markedly higher in PST rats. In rats with long-surviving grafts, overall peripheral blood chimerism was more than 5%, and both lymphocytes and granulocytes generated from the grafted spleen were stable. T-cell subsets in the recipient LEW rats varied according to the type of cells. CD4(+)CD8(+) subsets decreased in the GFP(+) cells and CD4(-)CD8(+) subsets increased in the GFP(-) (LEW) cells. CONCLUSION:We confirmed the combination effect of the grafted spleen on pancreatic graft survival. Donor lymphocytic and granulocytic lineages were generated in the recipients with long-surviving graft. It suggested that multilineage chimerism was often induced by the spleen graft and protected the pancreatic graft against rejection for a long period. |
| 巻・号 | 12(2) |
| ページ | 115-22 |
| 公開日 | 2004-1-1 |
| DOI | 10.1016/j.trim.2003.10.002 |
| PII | S0966-3274(03)00097-2 |
| PMID | 14967309 |
| MeSH | Animals Animals, Genetically Modified Bone Marrow / metabolism Cell Lineage Flow Cytometry Graft Rejection Graft Survival Granulocytes / cytology Granulocytes / metabolism* Green Fluorescent Proteins Luminescent Proteins / genetics Luminescent Proteins / metabolism* Lymphocytes / cytology Lymphocytes / metabolism* Male Models, Animal* Pancreas / cytology Pancreas / metabolism Pancreas Transplantation* Rats Rats, Wistar Spleen / cytology Spleen / metabolism Spleen / transplantation* T-Lymphocyte Subsets / cytology T-Lymphocyte Subsets / metabolism Transgenes / genetics Transplantation Chimera / genetics Transplantation Chimera / immunology Transplantation Chimera / metabolism* Transplantation, Homologous / immunology |
| IF | 1.624 |
| 引用数 | 6 |
| WOS 分野 | TRANSPLANTATION IMMUNOLOGY |
| リソース情報 | |
| ラット | W-Tg(CAG-GFP)184Ys(strainID=525) |