論文 - 詳細
| RRC ID | 11112 |
|---|---|
| 著者 | Boutros M, Ahringer J. |
| タイトル | The art and design of genetic screens: RNA interference. |
| ジャーナル | Nat Rev Genet |
| Abstract |
The remarkable gene knockdown technique of RNAi has opened exciting new avenues for genetic screens in model organisms and human cells. Here we describe the current state of the art for RNAi screening, and stress the importance of well-designed assays and of analytical approaches for large-scale screening experiments, from high-throughput screens using simplified homogenous assays to microscopy and whole-animal experiments. Like classical genetic screens in the past, the success of large-scale RNAi surveys depends on a careful development of phenotypic assays and their interpretation in a relevant biological context. |
| 巻・号 | 9(7) |
| ページ | 554-66 |
| 公開日 | 2008-7-1 |
| DOI | 10.1038/nrg2364 |
| PII | nrg2364 |
| PMID | 18521077 |
| MeSH | Animals Cell Line Genetic Techniques* Humans RNA Interference* Research Design |
| IF | 33.133 |
| 引用数 | 267 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 46 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| ショウジョウバエ | |