RRC ID 11122
著者 Leibfried A, Fricke R, Morgan MJ, Bogdan S, Bellaiche Y.
タイトル Drosophila Cip4 and WASp define a branch of the Cdc42-Par6-aPKC pathway regulating E-cadherin endocytosis.
ジャーナル Curr Biol
Abstract BACKGROUND:Integral to the function and morphology of the epithelium is the lattice of cell-cell junctions known as adherens junctions (AJs). AJ stability and plasticity relies on E-Cadherin exocytosis and endocytosis. A mechanism regulating E-Cadherin (E-Cad) exocytosis to the AJs has implicated proteins of the exocyst complex, but mechanisms regulating E-Cad endocytosis from the AJs remain less well understood.
RESULTS:Here we show that Cdc42, Par6, or aPKC loss of function is accompanied by the accumulation of apical E-Cad intracellular punctate structures and the disruption of AJs in Drosophila epithelial cells. These punctate structures derive from large and malformed endocytic vesicles that emanate from the AJs; a phenotype that is also observed upon blocking vesicle scission in dynamin mutant cells. We demonstrate that the Drosophila Cdc42-interacting protein 4 (Cip4) is a Cdc42 effector that interacts with Dynamin and the Arp2/3 activator WASp in Drosophila. Accordingly, Cip4, WASp, or Arp2/3 loss of function also results in defective E-Cadherin endocytosis.
CONCLUSION:Altogether our results show that Cdc42 functions with Par6 and aPKC to regulate E-Cad endocytosis and define Cip4 and WASp as regulators of the early E-Cad endocytic events in epithelial tissue.
巻・号 18(21)
ページ 1639-48
公開日 2008-11-11
DOI 10.1016/j.cub.2008.09.063
PII S0960-9822(08)01341-9
PMID 18976911
MeSH Actin-Related Protein 2-3 Complex / metabolism Actins / metabolism Adherens Junctions / metabolism* Animals Cadherins / metabolism* Carrier Proteins / metabolism Drosophila / metabolism Drosophila Proteins / metabolism* Dynamins / metabolism Endocytosis* Epithelial Cells / metabolism Protein Kinase C / metabolism* Wiskott-Aldrich Syndrome Protein / metabolism cdc42 GTP-Binding Protein / metabolism*
IF 9.601
引用数 162
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ 15015R-3