| RRC ID |
11130
|
| 著者 |
Zeitouni B, Sénatore S, Séverac D, Aknin C, Sémériva M, Perrin L.
|
| タイトル |
Signalling pathways involved in adult heart formation revealed by gene expression profiling in Drosophila.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
Drosophila provides a powerful system for defining the complex genetic programs that drive organogenesis. Under control of the steroid hormone ecdysone, the adult heart in Drosophila forms during metamorphosis by a remodelling of the larval cardiac organ. Here, we evaluated the extent to which transcriptional signatures revealed by genomic approaches can provide new insights into the molecular pathways that underlie heart organogenesis. Whole-genome expression profiling at eight successive time-points covering adult heart formation revealed a highly dynamic temporal map of gene expression through 13 transcript clusters with distinct expression kinetics. A functional atlas of the transcriptome profile strikingly points to the genomic transcriptional response of the ecdysone cascade, and a sharp regulation of key components belonging to a few evolutionarily conserved signalling pathways. A reverse genetic analysis provided evidence that these specific signalling pathways are involved in discrete steps of adult heart formation. In particular, the Wnt signalling pathway is shown to participate in inflow tract and cardiomyocyte differentiation, while activation of the PDGF-VEGF pathway is required for cardiac valve formation. Thus, a detailed temporal map of gene expression can reveal signalling pathways responsible for specific developmental programs and provides here substantial grasp into heart formation.
|
| 巻・号 |
3(10)
|
| ページ |
1907-21
|
| 公開日 |
2007-10-1
|
| DOI |
10.1371/journal.pgen.0030174
|
| PII |
07-PLGE-RA-0335
|
| PMID |
17937502
|
| PMC |
PMC2014791
|
| MeSH |
Animals
Drosophila Proteins / metabolism
Drosophila melanogaster
Ecdysone / metabolism
Gene Expression Profiling*
Gene Expression Regulation
Genome
Heart / physiology*
Kinetics
Myocardium / metabolism
Oligonucleotide Array Sequence Analysis
Platelet-Derived Growth Factor / metabolism
Signal Transduction
Transcription, Genetic
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
5.175
|
| 引用数 |
53
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| ショウジョウバエ |
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