Reference - Detail
| RRC ID | 11142 |
|---|---|
| Author | Sasaki N, Yoshida H, Fuwa TJ, Kinoshita-Toyoda A, Toyoda H, Hirabayashi Y, Ishida H, Ueda R, Nishihara S. |
| Title | Drosophila beta 1,4-N-acetylgalactosaminyltransferase-A synthesizes the LacdiNAc structures on several glycoproteins and glycosphingolipids. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
The GalNAcbeta1,4GlcNAc (LacdiNAc or LDN) structure is a more common structural feature in invertebrate glycoconjugates when compared with the Galbeta1,4GlcNAc structure. Recently, beta1,4-N-acetylgalactosaminyltransferase (beta4GalNAcT) was identified in some invertebrates including Drosophila. However, the LDN structure has not been reported in Drosophila, and the biological function of LDN remains to be determined. In this study, we examined acceptor substrate specificity of Drosophila beta4GalNAcTA by using some N- and O-glycans on glycoproteins and neutral glycosphingolipids (GSLs). GalNAc was efficiently transferred toward N-glycans, O-glycans, and the arthro-series GSLs. Moreover, we showed that dbeta4GalNAcTA contributed to the synthesis of the LDN structure in vivo. The dbeta4GalNAcTA mRNA was highly expressed in the developmental and adult neuronal tissues. Thus, these results suggest that dbeta4GalNAcTA acts on the terminal GlcNAc residue of some glycans for the synthesis of LDN, and the LDN structure may play a role in the physiological or neuronal development of Drosophila. |
| Volume | 354(2) |
| Pages | 522-7 |
| Published | 2007-3-9 |
| DOI | 10.1016/j.bbrc.2007.01.015 |
| PII | S0006-291X(07)00041-1 |
| PMID | 17239818 |
| MeSH | Animals Carbohydrate Sequence Disaccharides / biosynthesis* Disaccharides / chemistry Drosophila Proteins / biosynthesis* Drosophila Proteins / chemistry Drosophila Proteins / physiology Glycoproteins / biosynthesis* Glycosphingolipids / biosynthesis* Lactose / analogs & derivatives* Lactose / biosynthesis Lactose / chemistry Molecular Sequence Data N-Acetylgalactosaminyltransferases / chemistry N-Acetylgalactosaminyltransferases / physiology* |
| IF | 2.985 |
| Times Cited | 16 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | |