RRC ID 1115
著者 Hardy SP, Nakano M, Iida T.
タイトル Single channel evidence for innate pore-formation by Vibrio parahaemolyticus thermostable direct haemolysin (TDH) in phospholipid bilayers.
ジャーナル FEMS Microbiol Lett
Abstract Vibrio parahaemolyticus thermostable direct haemolysin (TDH) is widely considered to be a pore-forming toxin. The protein has no significant homology to other known pore-forming toxins and its mechanism of action in vivo remains undefined. We demonstrate single channel pore-forming activity of V. parahaemolyticus TDH in planar lipid bilayers. Channel conductance ranged between 30-450 pS in 0.5 M KCl with a calculated cation selectivity (P(K)/P(Cl)) of 2.7. Channels were formed in NaCl and choline-Cl with and without cholesterol present and in the presence of neutral or negatively charged phospholipids. Zinc ions did not block pore formation. Whilst various techniques have previously suggested that TDH is a pore-forming toxin, the data in this study provide direct single channel evidence and indicate several features of pore formation in synthetic phospholipid membranes.
巻・号 240(1)
ページ 81-5
公開日 2004-11-1
DOI 10.1016/j.femsle.2004.09.015
PII S0378-1097(04)00678-0
PMID 15500983
MeSH Amino Acid Sequence Anions / metabolism Bacterial Toxins Cations / metabolism Hemolysin Proteins / chemistry* Hemolysin Proteins / physiology* Lipid Bilayers / chemistry Membrane Potentials Molecular Sequence Data Patch-Clamp Techniques Phospholipids / chemistry Protein Structure, Quaternary Protein Structure, Secondary Vibrio parahaemolyticus / physiology*
IF 1.987
引用数 11
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 V. parahaemolyticus?