| RRC ID |
11152
|
| 著者 |
Drapeau MD, Cyran SA, Viering MM, Geyer PK, Long AD.
|
| タイトル |
A cis-regulatory sequence within the yellow locus of Drosophila melanogaster required for normal male mating success.
|
| ジャーナル |
Genetics
|
| Abstract |
Drosophila melanogaster males perform a courtship ritual consisting of a series of dependent fixed-action patterns. The yellow (y) gene is required for normal male courtship behavior and subsequent mating success. To better characterize the requirement for y in the manifestation of innate male sexual behavior, we measured the male mating success (MMS) of 12 hypomorphic y mutants and matched-outbred-background controls using a y+ rescue element on a freely segregating minichromosome. We found that 4 hypomorphs significantly reduced MMS to varying degrees. Reduced MMS was largely independent of adult pigmentation patterns. These mutations defined a 300-bp regulatory region upstream of the transcription start, the mating-success regulatory sequence (MRS), whose function is required for normal MMS. Visualization of gene action via GFP and a Yellow antibody suggests that the MRS directs y transcription in a small number of cells in the third instar CNS, the developmental stage previously implicated in the role of y with regard to male courtship behavior. The presence of Yellow protein in these cells positively correlates with MMS in a subset of mutants. The MRS contains a regulatory sequence controlling larval pigmentation and a 35-bp sequence that is highly conserved within the genus Drosophila and is predicted to bind known transcription factors.
|
| 巻・号 |
172(2)
|
| ページ |
1009-30
|
| 公開日 |
2006-2-1
|
| DOI |
10.1534/genetics.105.045666
|
| PII |
genetics.105.045666
|
| PMID |
16272418
|
| PMC |
PMC1456202
|
| MeSH |
Animals
Base Sequence
Courtship
Crosses, Genetic
Drosophila Proteins / genetics*
Drosophila Proteins / physiology
Gene Expression Regulation, Developmental / physiology*
Genetic Markers
Genetic Variation
Larva / genetics
Male
Molecular Sequence Data
Pigmentation / genetics
Sexual Behavior, Animal / physiology*
Transcription Factors / physiology
|
| IF |
4.015
|
| 引用数 |
43
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| ショウジョウバエ |
|
